Title:
NUCLEIC ACID ANALYSIS DEVICE
Document Type and Number:
WIPO Patent Application WO/2014/034275
Kind Code:
A1
Abstract:
Provided is a nucleic acid analysis device which can decode a long nucleotide sequence in a DNA sequence with a high parallel processing and high accuracy. The nucleotide analysis device according to the present invention is equipped with a flow cell onto which at least two pieces of DNA fragment aggregates each composed of an aggregate of at least two DNA fragments having the identical nucleotide sequence to each other are immobilized and at least a part of which is made from a transparent material, an irradiation section which irradiates a site at which the DNA fragment aggregates are immobilized, a lens which collects fluorescent light, and a light detection element which detects the collected light, said method being characterized in that a solution containing only dATP among four nucleotides each having a fluorescence-modified phosphate group terminal, a solution containing only dCTP among the four nucleotides each having a fluorescence-modified phosphate group terminal, a solution containing only dGTP among the four nucleotides each having a fluorescence-modified phosphate group terminal, a solution containing only dTTP among the four nucleotides each having a fluorescence-modified phosphate group terminal, and a buffer solution for washing the nucleotides are supplied sequentially to the site at which the DNA fragment aggregates are immobilized.
Inventors:
HAGA TAKANOBU (JP)
KODAMA YOSHITAKA (JP)
SHOJI TOMOHIRO (JP)
MURAMATSU TAKAMICHI (JP)
YAMAMOTO SHUHEI (JP)
KODAMA YOSHITAKA (JP)
SHOJI TOMOHIRO (JP)
MURAMATSU TAKAMICHI (JP)
YAMAMOTO SHUHEI (JP)
Application Number:
PCT/JP2013/068588
Publication Date:
March 06, 2014
Filing Date:
July 08, 2013
Export Citation:
Assignee:
HITACHI HIGH TECH CORP (JP)
International Classes:
C12M1/00; C12N15/09; G01N21/64; G01N21/78; G01N33/53; G01N33/543; G01N37/00
Domestic Patent References:
| WO2012111366A1 | 2012-08-23 |
Foreign References:
| JP2009514518A | 2009-04-09 |
Other References:
ATSUSHI TOYOTA ET AL.: "Jisedai Sequencer ni yoru Genome Kaidoku to Kongo no Doko", EXPERIMENTAL MEDICINE, vol. 27, no. 12, 2009, pages 113 - 119
SUN C. ET AL.: "De novo sequencing and analysis of the American ginseng root transcriptome using a GS FLX Titanium platform to discover putative genes involved in ginsenoside biosynthesis.", BMC GENOMICS, vol. 11, no. 262, 2010, pages 1 - 12, XP021072582
CHUN J. ET AL.: "The analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454 GS FLX Titanium pyrosequencer.", BMC MICROBIOLOGY, vol. 10, no. 101, 2010, pages 1 - 8, XP021072985
EID J. ET AL.: "Real-Time DNA Sequencing from Single Polymerase Molecules.", SCIENCE, vol. 323, 2009, pages 133 - 138, XP055096175
TRAN B. ET AL.: "Cancer Genomics: Technology, Discovery, and Translation.", J CLIN ONCOL., vol. 30, no. 6, February 2012 (2012-02-01), pages 647 - 660, XP055188691
PASKO D. A. ET AL.: "Origins of the E. coli Strain Causing an Outbreak of Hemolytic-Uremic Syndrome in Germany.", N ENGL J MED., vol. 365, no. 8, 2011, pages 709 - 717, XP055030405
LIU S. ET AL.: "Optimization of High-Speed DNA Sequencing on Microfabricated Capillary Electrophoresis Channels.", ANAL CHEM., vol. 71, no. 3, 1999, pages 566 - 573, XP002204764
D. R. BENTLEY ET AL.: "Accurate whole human genome sequencing using reversible terminator chemistry", NATURE, vol. 456, 2008, pages 53 - 59
M. MARGULIES ET AL.: "enome sequencing in microfabricate high-density picolitre reactors", NATURE, vol. 437, 2005, pages 376 - 380
BRENT A. MULDER ET AL., NUCLEIC ACIDS RESEARCH, vol. 33, no. 15, 2005, pages 4865 - 4873
JONAS KORLACH ET AL., NUCLEOSIDES, NUCLEOTIDES.AND NUCLEIC. ACIDS, vol. 27, 2008, pages 1072 - 1083
SHAORONG LIU ET AL., ANAL. CHEM., vol. 71, 1999, pages 566 - 573
MAKIO TOKUNAGA ET AL., NAT. METHODS, vol. 5, 2008, pages 159 - 161
YASUSHI SAKO, MOLECULAR SYSTEMS BIOLOGY, 2006, pages 56
PETER KNER ET AL., NATURE METHODS, vol. 6, no. 5, 2009, pages 339 - 342
R. DRMANAC ET AL., SCIENCE, vol. 327, 2010, pages 78 - 81
See also references of EP 2891709A4
SUN C. ET AL.: "De novo sequencing and analysis of the American ginseng root transcriptome using a GS FLX Titanium platform to discover putative genes involved in ginsenoside biosynthesis.", BMC GENOMICS, vol. 11, no. 262, 2010, pages 1 - 12, XP021072582
CHUN J. ET AL.: "The analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454 GS FLX Titanium pyrosequencer.", BMC MICROBIOLOGY, vol. 10, no. 101, 2010, pages 1 - 8, XP021072985
EID J. ET AL.: "Real-Time DNA Sequencing from Single Polymerase Molecules.", SCIENCE, vol. 323, 2009, pages 133 - 138, XP055096175
TRAN B. ET AL.: "Cancer Genomics: Technology, Discovery, and Translation.", J CLIN ONCOL., vol. 30, no. 6, February 2012 (2012-02-01), pages 647 - 660, XP055188691
PASKO D. A. ET AL.: "Origins of the E. coli Strain Causing an Outbreak of Hemolytic-Uremic Syndrome in Germany.", N ENGL J MED., vol. 365, no. 8, 2011, pages 709 - 717, XP055030405
LIU S. ET AL.: "Optimization of High-Speed DNA Sequencing on Microfabricated Capillary Electrophoresis Channels.", ANAL CHEM., vol. 71, no. 3, 1999, pages 566 - 573, XP002204764
D. R. BENTLEY ET AL.: "Accurate whole human genome sequencing using reversible terminator chemistry", NATURE, vol. 456, 2008, pages 53 - 59
M. MARGULIES ET AL.: "enome sequencing in microfabricate high-density picolitre reactors", NATURE, vol. 437, 2005, pages 376 - 380
BRENT A. MULDER ET AL., NUCLEIC ACIDS RESEARCH, vol. 33, no. 15, 2005, pages 4865 - 4873
JONAS KORLACH ET AL., NUCLEOSIDES, NUCLEOTIDES.AND NUCLEIC. ACIDS, vol. 27, 2008, pages 1072 - 1083
SHAORONG LIU ET AL., ANAL. CHEM., vol. 71, 1999, pages 566 - 573
MAKIO TOKUNAGA ET AL., NAT. METHODS, vol. 5, 2008, pages 159 - 161
YASUSHI SAKO, MOLECULAR SYSTEMS BIOLOGY, 2006, pages 56
PETER KNER ET AL., NATURE METHODS, vol. 6, no. 5, 2009, pages 339 - 342
R. DRMANAC ET AL., SCIENCE, vol. 327, 2010, pages 78 - 81
See also references of EP 2891709A4
Attorney, Agent or Firm:
INOUE Manabu et al. (JP)
Manabu Inoue (JP)
Manabu Inoue (JP)
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