NUCLEIC ACID ANALYSIS METHOD

Title:

NUCLEIC ACID ANALYSIS METHOD

Document Type and Number:

WIPO Patent Application WO/2013/021841

Kind Code:

A1

Abstract:

An embodiment relates to a nucleic acid analysis method for a sample. The nucleic acid analysis method comprises subjecting the sample to an amplification reaction using a first primer and a second primer, reacting an amplification reaction product with a nucleic acid probe, determining the occurrence of hybridization and/or the amount of the hybridization, and determining the presence of a target nucleic acid and/or the abundance of the target nucleic acid in the sample. The amplification product produced using the first primer and/or the second primer forms a stem-loop structure. The nucleic acid analysis on the sample can be achieved by detecting the occurrence of the hybridization between a nucleic acid probe complementary to the sequence for a loop structure and the amplification product and/or abundance of the hybridization.

Inventors:

NAKAMURA NAOKO (JP)
HASHIMOTO KOJI (JP)
HASHIMOTO MICHIE (JP)
GEMMA NOBUHIRO (JP)
NIKAIDO MASARU (JP)

Application Number:

PCT/JP2012/069170

Publication Date:

February 14, 2013

Filing Date:

July 27, 2012

Export Citation:

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Assignee:

TOSHIBA KK (JP)
NAKAMURA NAOKO (JP)
HASHIMOTO KOJI (JP)
HASHIMOTO MICHIE (JP)
GEMMA NOBUHIRO (JP)
NIKAIDO MASARU (JP)

International Classes:

C12Q1/68; C12N15/09

Foreign References:

JP2010520756A	2010-06-17
US20100143898A1	2010-06-10
US20110212846A1	2011-09-01

Other References: