Title:
NUCLEIC ACID CALIBRATION STANDARDS
Document Type and Number:
WIPO Patent Application WO/2014/145621
Kind Code:
A3
Abstract:
The invention relates to synthetic nucleic acids and in particular, complementary pairs and their use as calibration standards for various techniques used to measure the interaction of biological molecules. The nucleotide sequence of the nucleic acids are non-naturally occurring and the single-stranded nucleic acids are essentially lacking in secondary structure, that is, the stability of hybridization between a single stranded nucleic acid and its complement is greater than any intra-strand pair binding. Characteristics of a binding pair can be used to standardize data across experiments, across instruments and across platforms.
Inventors:
AGRIS PAUL F (US)
HALVORSEN KEN (US)
HALVORSEN KEN (US)
Application Number:
PCT/US2014/030416
Publication Date:
November 13, 2014
Filing Date:
March 17, 2014
Export Citation:
Assignee:
UNIV NEW YORK STATE RES FOUND (US)
International Classes:
C12Q1/68
Other References:
SALIM N N ET AL: "Isothermal titration calorimetry of RNA", METHODS, ACADEMIC PRESS, vol. 47, no. 3, 1 March 2009 (2009-03-01), pages 198 - 205, XP025917815, ISSN: 1046-2023, [retrieved on 20090209], DOI: 10.1016/J.YMETH.2008.09.003
JENNIFER C. TAKACH ET AL: "Salt-Dependent Heat Capacity Changes for RNA Duplex Formation", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 126, no. 21, 1 June 2004 (2004-06-01), pages 6530 - 6531, XP055119575, ISSN: 0002-7863, DOI: 10.1021/ja0316263
PETER J. MIKULECKY ET AL: "Heat Capacity Changes Associated with DNA Duplex Formation: Salt- and Sequence-Dependent Effects +", BIOCHEMISTRY, vol. 45, no. 2, 1 January 2006 (2006-01-01), pages 604 - 616, XP055119568, ISSN: 0006-2960, DOI: 10.1021/bi0517178
ANONYMOUS: "Oligo dT 12-18 Primer Certificate of Analysis", 1 November 2005 (2005-11-01), XP055119937, Retrieved from the Internet [retrieved on 20140523]
"Protein Engineering for Therapeutics, Part B", vol. 468, 1 January 2009, ELSEVIER, ISBN: 978-0-12-396962-0, ISSN: 0076-6879, article ANDREW L. FEIG: "Studying RNA-RNA and RNA-Protein Interactions by Isothermal Titration Calorimetry", pages: 409 - 422, XP055119472, DOI: 10.1016/S0076-6879(09)68019-8
JENNIFER C. TAKACH ET AL: "Salt-Dependent Heat Capacity Changes for RNA Duplex Formation", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 126, no. 21, 1 June 2004 (2004-06-01), pages 6530 - 6531, XP055119575, ISSN: 0002-7863, DOI: 10.1021/ja0316263
PETER J. MIKULECKY ET AL: "Heat Capacity Changes Associated with DNA Duplex Formation: Salt- and Sequence-Dependent Effects +", BIOCHEMISTRY, vol. 45, no. 2, 1 January 2006 (2006-01-01), pages 604 - 616, XP055119568, ISSN: 0006-2960, DOI: 10.1021/bi0517178
ANONYMOUS: "Oligo dT 12-18 Primer Certificate of Analysis", 1 November 2005 (2005-11-01), XP055119937, Retrieved from the Internet
"Protein Engineering for Therapeutics, Part B", vol. 468, 1 January 2009, ELSEVIER, ISBN: 978-0-12-396962-0, ISSN: 0076-6879, article ANDREW L. FEIG: "Studying RNA-RNA and RNA-Protein Interactions by Isothermal Titration Calorimetry", pages: 409 - 422, XP055119472, DOI: 10.1016/S0076-6879(09)68019-8
Attorney, Agent or Firm:
DIAS, Kathy Smith (5 Columbia CircleAlbany, New York, US)
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