Title:
NUCLEIC ACID CONSTRUCT FOR ACHIEVING MODULAR LOADING OF ENGINEERED EVS FUNCTIONAL PROTEIN AND USE OF SAID CONSTRUCT
Document Type and Number:
WIPO Patent Application WO/2023/222105
Kind Code:
A1
Abstract:
Provided are a nucleic acid construct for achieving modular loading of an engineered EVs functional protein and use of said construct. A modular design principle is adopted, specific modules are selected for combination, a mutation sequence is optimized and screened to obtain an improved nucleic acid construct, and finally, the engineered EVs modularly loaded with functional protein is converted and expressed. The product has the following advantages that: I) the modular loading of the engineered EVs functional protein is achieved; 2) the loading density and the loading efficiency of the protein inside and outside an EVs membrane are improved; and 3) a specific support sequence module is selected to avoid enzyme digestion. The construct is used to prepare the engineered EVs, so that the modular loading of the functional protein can be achieved, the loading efficiency is improved, and the construct has a wide clinical application value and market prospect.
Inventors:
WANG YI (CN)
SONG HAIFENG (CN)
DONG YANAN (CN)
XUE MIAOMIAO (CN)
LI YANFANG (CN)
ZHANG JING (CN)
ZHOU WEI (CN)
XUE LIJIA (CN)
SONG HAIFENG (CN)
DONG YANAN (CN)
XUE MIAOMIAO (CN)
LI YANFANG (CN)
ZHANG JING (CN)
ZHOU WEI (CN)
XUE LIJIA (CN)
Application Number:
PCT/CN2023/095183
Publication Date:
November 23, 2023
Filing Date:
May 19, 2023
Export Citation:
Assignee:
PANEXO BIOSCIENCES CO LTD (CN)
International Classes:
C12N15/85; A61K38/00; A61K39/00; C07K14/00; C12N15/11; C12N15/63
Domestic Patent References:
| WO2021030777A1 | 2021-02-18 | |||
| WO2021152115A1 | 2021-08-05 |
Foreign References:
| CN113286603A | 2021-08-20 | |||
| CN111511384A | 2020-08-07 | |||
| CN113226355A | 2021-08-06 |
Other References:
O’BRIEN KILLIAN, UGHETTO STEFANO, MAHJOUM SHADI, NAIR ANIL V., BREAKEFIELD XANDRA O.: "Uptake, functionality, and re-release of extracellular vesicle-encapsulated cargo", CELL REPORTS, ELSEVIER INC, US, vol. 39, no. 2, 1 April 2022 (2022-04-01), US , pages 110651, XP093109274, ISSN: 2211-1247, DOI: 10.1016/j.celrep.2022.110651
ANDREU ZORAIDA, YáñEZ-Mó MARÃA: "Tetraspanins in Extracellular Vesicle Formation and Function", FRONTIERS IN IMMUNOLOGY, FRONTIERS MEDIA, LAUSANNE, CH, vol. 5, 16 September 2014 (2014-09-16), Lausanne, CH , pages 442, XP093109276, ISSN: 1664-3224, DOI: 10.3389/fimmu.2014.00442
ANDREU ZORAIDA, YáñEZ-Mó MARÃA: "Tetraspanins in Extracellular Vesicle Formation and Function", FRONTIERS IN IMMUNOLOGY, FRONTIERS MEDIA, LAUSANNE, CH, vol. 5, 16 September 2014 (2014-09-16), Lausanne, CH , pages 442, XP093109276, ISSN: 1664-3224, DOI: 10.3389/fimmu.2014.00442
Attorney, Agent or Firm:
UNITALEN ATTORNEYS AT LAW CO., LTD. (CN)
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