Title:
NUCLEIC ACID CONSTRUCT AND METHOD OF TRANSFERRING MUTATION INTO TARGET REGION BY USING THE SAME
Document Type and Number:
WIPO Patent Application WO/2006/064813
Kind Code:
A1
Abstract:
It is intended to provide a nucleic acid construct by which a mutation can be transferred into a target region of a double-stranded DNA in a cell. To achieve this object, a nucleic acid construct, which comprises a single-stranded DNA having a base sequence derived from a base sequence in the sense strand side region or the antisense strand side region of a target region located in a double-stranded DNA in a cell by deletion, substitution, insertion or addition of one or more bases and having a 90% or more homology with the base sequence of the sense strand side region or the antisense strand side region as described above and another single-stranded DNA containing a base sequence complementary to the base sequence of a part of the single-stranded DNA as described above and having been annealed to the single-stranded DNA as described above, is provided.
Inventors:
KAMIYA HIROYUKI
HARASHIMA HIDEYOSHI
TSUCHIYA HIROYUKI
HARASHIMA HIDEYOSHI
TSUCHIYA HIROYUKI
Application Number:
PCT/JP2005/022890
Publication Date:
June 22, 2006
Filing Date:
December 13, 2005
Export Citation:
Assignee:
UNIV HOKKAIDO NAT UNIV CORP (JP)
KAMIYA HIROYUKI
HARASHIMA HIDEYOSHI
TSUCHIYA HIROYUKI
KAMIYA HIROYUKI
HARASHIMA HIDEYOSHI
TSUCHIYA HIROYUKI
International Classes:
C12N15/09; A61K31/711; A61K48/00; A61P43/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10
Domestic Patent References:
| WO2005075657A1 | 2005-08-18 |
Other References:
TSUCHIYA H. ET AL.: "Phagemid Plasmid kara Chosei shita DNA Danpen ni yoru Idenshi Shufuku Koritsu no Kojo", ANNUAL MEETING OF THE MOLECULAR BIOLOGY SCIENTY OF JAPAN PROGRAM KOEN YOSHISHU, vol. 27TH, 25 November 2004 (2004-11-25), pages 884, 3PA-367, XP003000440
MAZIN A.V. ET AL.: "Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing", EMBO J., vol. 19, no. 5, 2000, pages 1148 - 1156, XP003000441
LIANG L. ET AL.: "Optimizing the delivery systems of chimeric RNA.DNA oligonucleotides. Beyong general oligonucleotide transfer", EUR. J. BIOCHEM., vol. 269, 2002, pages 5753 - 5758, XP003000442
TSUCHIYA H. ET AL.: "Ipponsa DNA Danpen ni yoru Idenshi Shufuku Koritsu ni Eikyo o Ataeru Inshi", ANNUAL MEETING OF THE MOLECULAR BIOLOGY SCIENTY OF JAPAN KOEN YOSHISHU, vol. 28TH, 25 November 2005 (2005-11-25), pages 755, 3P-1146, XP003000443
TSUCHIYA H. ET AL.: "Increased SFHR gene correction efficiency with sense single-stranded DNA", J. GENE MED., vol. 7, no. 4, April 2005 (2005-04-01), pages 486 - 493, XP003000444
MAZIN A.V. ET AL.: "Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing", EMBO J., vol. 19, no. 5, 2000, pages 1148 - 1156, XP003000441
LIANG L. ET AL.: "Optimizing the delivery systems of chimeric RNA.DNA oligonucleotides. Beyong general oligonucleotide transfer", EUR. J. BIOCHEM., vol. 269, 2002, pages 5753 - 5758, XP003000442
TSUCHIYA H. ET AL.: "Ipponsa DNA Danpen ni yoru Idenshi Shufuku Koritsu ni Eikyo o Ataeru Inshi", ANNUAL MEETING OF THE MOLECULAR BIOLOGY SCIENTY OF JAPAN KOEN YOSHISHU, vol. 28TH, 25 November 2005 (2005-11-25), pages 755, 3P-1146, XP003000443
TSUCHIYA H. ET AL.: "Increased SFHR gene correction efficiency with sense single-stranded DNA", J. GENE MED., vol. 7, no. 4, April 2005 (2005-04-01), pages 486 - 493, XP003000444
Attorney, Agent or Firm:
Hayakawa, Yuzi (Suite 501 Hikawa-Annex No. 2, 9-5, Akasaka 6-chom, Minato-ku Tokyo, JP)
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