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Title:
NUCLEIC ACID DETECTION METHOD, AND DEVICE AND KIT FOR USE IN SAME
Document Type and Number:
WIPO Patent Application WO/2012/121225
Kind Code:
A1
Abstract:
The invention addresses the problem of preventing the problems of cost and handleability, and the contamination or exposure of a sample, which are problematic in the analysis of an amplified nucleic acid. According to the invention, a sample containing a nucleic acid and a nucleic acid detection reagent are mixed in a closed system. At this time, the nucleic acid is mixed with the detection reagent after being amplified in the same closed system without the system being opened. As a means therefor, for example, a device in which the detection reagent is shielded by a coating material or the like is used.

Inventors:
MIYAMOTO SHIGEHIKO (JP)
TOMONO JUN (JP)
TAKAHASHI KOJI (JP)
SANO SOTARO (JP)
JIKIHARA TAKAAKI (JP)
Application Number:
PCT/JP2012/055601
Publication Date:
September 13, 2012
Filing Date:
March 05, 2012
Export Citation:
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Assignee:
KANEKA CORP (JP)
MIYAMOTO SHIGEHIKO (JP)
TOMONO JUN (JP)
TAKAHASHI KOJI (JP)
SANO SOTARO (JP)
JIKIHARA TAKAAKI (JP)
International Classes:
C12Q1/68; C12M1/34; G01N33/50; C12N15/09
Domestic Patent References:
WO2010106997A12010-09-23
Foreign References:
JP2009201458A2009-09-10
JP4683811B22011-05-18
JP2003038161A2003-02-12
JP2009201458A2009-09-10
Other References:
LOOPAMP TANJUN HERPES VIRUS (HSV-1/2) KENSHUTSU SHIYAKU KIT, MANUAL, 2010, XP008171202
OPTIGENE-SHA TOON ZOFUKU KEIKO SOKUTEI SOCHI GENIEII JIZEN YOYAKU CAMPAIGN, 2010, XP055138317
"Molecular Cloning", vol. 1, 1989, pages: 6.15
NATURE PROTOCOLS, vol. 3, no. 5, 2008, pages 877 - 882
See also references of EP 2682480A4
Attorney, Agent or Firm:
YASUTOMI & Associates (JP)
Patent business corporation Yasutomi international patent firm (JP)
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Claims: