Title:
NUCLEIC ACID DETECTION METHOD, AND DEVICE AND KIT FOR USE IN SAME
Document Type and Number:
WIPO Patent Application WO/2012/121225
Kind Code:
A1
Abstract:
The invention addresses the problem of preventing the problems of cost and handleability, and the contamination or exposure of a sample, which are problematic in the analysis of an amplified nucleic acid. According to the invention, a sample containing a nucleic acid and a nucleic acid detection reagent are mixed in a closed system. At this time, the nucleic acid is mixed with the detection reagent after being amplified in the same closed system without the system being opened. As a means therefor, for example, a device in which the detection reagent is shielded by a coating material or the like is used.
Inventors:
MIYAMOTO SHIGEHIKO (JP)
TOMONO JUN (JP)
TAKAHASHI KOJI (JP)
SANO SOTARO (JP)
JIKIHARA TAKAAKI (JP)
TOMONO JUN (JP)
TAKAHASHI KOJI (JP)
SANO SOTARO (JP)
JIKIHARA TAKAAKI (JP)
Application Number:
PCT/JP2012/055601
Publication Date:
September 13, 2012
Filing Date:
March 05, 2012
Export Citation:
Assignee:
KANEKA CORP (JP)
MIYAMOTO SHIGEHIKO (JP)
TOMONO JUN (JP)
TAKAHASHI KOJI (JP)
SANO SOTARO (JP)
JIKIHARA TAKAAKI (JP)
MIYAMOTO SHIGEHIKO (JP)
TOMONO JUN (JP)
TAKAHASHI KOJI (JP)
SANO SOTARO (JP)
JIKIHARA TAKAAKI (JP)
International Classes:
C12Q1/68; C12M1/34; G01N33/50; C12N15/09
Domestic Patent References:
WO2010106997A1 | 2010-09-23 |
Foreign References:
JP2009201458A | 2009-09-10 | |||
JP4683811B2 | 2011-05-18 | |||
JP2003038161A | 2003-02-12 | |||
JP2009201458A | 2009-09-10 |
Other References:
LOOPAMP TANJUN HERPES VIRUS (HSV-1/2) KENSHUTSU SHIYAKU KIT, MANUAL, 2010, XP008171202
OPTIGENE-SHA TOON ZOFUKU KEIKO SOKUTEI SOCHI GENIEII JIZEN YOYAKU CAMPAIGN, 2010, XP055138317
"Molecular Cloning", vol. 1, 1989, pages: 6.15
NATURE PROTOCOLS, vol. 3, no. 5, 2008, pages 877 - 882
See also references of EP 2682480A4
OPTIGENE-SHA TOON ZOFUKU KEIKO SOKUTEI SOCHI GENIEII JIZEN YOYAKU CAMPAIGN, 2010, XP055138317
"Molecular Cloning", vol. 1, 1989, pages: 6.15
NATURE PROTOCOLS, vol. 3, no. 5, 2008, pages 877 - 882
See also references of EP 2682480A4
Attorney, Agent or Firm:
YASUTOMI & Associates (JP)
Patent business corporation Yasutomi international patent firm (JP)
Patent business corporation Yasutomi international patent firm (JP)
Download PDF:
Claims:
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