Title:
NUCLEIC ACID DETECTION METHOD, NUCLEIC ACID QUANTITATIVE DETERMINATION METHOD, NUCLEIC ACID BASE SEQUENCE IDENTIFICATION METHOD, NUCLEIC ACID MUTATION OR POLYMORPHISM IDENTIFICATION METHOD, NUCLEIC ACID DETECTION KIT, AND REACTION CHIP
Document Type and Number:
WIPO Patent Application WO/2016/194823
Kind Code:
A1
Abstract:
The present invention pertains to a method for detecting a target nucleic acid using a catalytic nucleic acid, wherein it is possible to improve the catalytic nucleic acid reaction efficiency and to achieve a continuous reaction under isothermal conditions. A nucleic acid detection method including a reaction step for obtaining a first nucleic acid product by hybridizing a first nucleic acid, which is a substrate, to a first binding site of a catalytic nucleic acid equipped with an active site having a catalytic action, and a detection step for detecting the first nucleic acid product and/or the unreacted first nucleic acid. Hybridization is conducted in the presence of a cationic comb polymer.
Inventors:
KITANO SHIRO (JP)
MARUYAMA ATSUSHI (JP)
SHIMADA NAOHIKO (JP)
MARUYAMA ATSUSHI (JP)
SHIMADA NAOHIKO (JP)
Application Number:
PCT/JP2016/065751
Publication Date:
December 08, 2016
Filing Date:
May 27, 2016
Export Citation:
Assignee:
TOPPAN PRINTING CO LTD (JP)
TOKYO INST TECH (JP)
TOKYO INST TECH (JP)
International Classes:
C12Q1/68; C12N15/09; C12Q1/25; G01N33/50; G01N33/53
Domestic Patent References:
| WO2013077391A1 | 2013-05-30 |
Foreign References:
| JP2012185000A | 2012-09-27 | |||
| JP2011214945A | 2011-10-27 |
Other References:
TOMOYA KOYANAGI ET AL.: "DNAzyme.Cationic Kobunshi ni yoru Kakusan Kenshutsuho", DAI 24 KAI INTELLIGENT ZAIRYO·SYSTEM SYMPOSIUM, 19 January 2015 (2015-01-19), pages 14, XP009507681
GAO, JUEYUAN ET AL.: "Cationic comb-type copolymer boosting DNAzyme catalyzed RNA cleavage reaction", POLYMER PREPRINTS, vol. 62, no. 2, 2013, Japan, pages 4863 - 4864, XP009507678
GAO, JUEYUAN ET AL.: "Cationic comb-type copolymer enhances MNAzyme activity for nucleic acid detection", THE 40TH INTERNATIONAL SYMPOSIUM ON NUCLEIC ACIDS CHEMISTRY; LIST OF POSTER PRESENTATIONS, 2013, pages 107, XP055503340, Retrieved from the Internet
See also references of EP 3305913A4
GAO, JUEYUAN ET AL.: "Cationic comb-type copolymer boosting DNAzyme catalyzed RNA cleavage reaction", POLYMER PREPRINTS, vol. 62, no. 2, 2013, Japan, pages 4863 - 4864, XP009507678
GAO, JUEYUAN ET AL.: "Cationic comb-type copolymer enhances MNAzyme activity for nucleic acid detection", THE 40TH INTERNATIONAL SYMPOSIUM ON NUCLEIC ACIDS CHEMISTRY; LIST OF POSTER PRESENTATIONS, 2013, pages 107, XP055503340, Retrieved from the Internet
See also references of EP 3305913A4
Attorney, Agent or Firm:
SUZUKI Shirou et al. (JP)
Shiro Suzuki (JP)
Shiro Suzuki (JP)
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