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Title:
NUCLEIC ACID ELEMENT CANDIDATE MOLECULE AND METHOD FOR SCREENING NUCLEIC ACID ELEMENTS FOR TARGET ANALYSIS USING SAME
Document Type and Number:
WIPO Patent Application WO/2014/136560
Kind Code:
A1
Abstract:
Provided are: a nucleic acid element candidate molecule used in the screening of nucleic acid elements for target analysis; and a new screening method for nucleic acid elements for target analysis using the nucleic acid element candidate molecule. The candidate molecule is for screening nucleic acid elements for target analysis, and is characterized by being a single-chain nucleic acid in the belowmentioned (I): (I) A single-chain nucleic acid such that a catalytic sequence (D), a blocking sequence (B), and a binding sequence (A) that binds to a target are joined in the given order, the blocking sequence (B) complements a partial region (Dp) in the catalytic sequence (D), and the terminal region (Ab) at the blocking sequence (B) side of the binding sequence (A) complements a neighboring region (Df) of the partial region (Dp) in the catalytic sequence (D), and complements the terminal region (Af) at the reverse side from the blocking sequence (B) side of the binding sequence (A).

Inventors:
AKITOMI JOU (JP)
KANEKO NAOTO (JP)
HORII KATSUNORI (JP)
Application Number:
JP2014/053664
Publication Date:
September 12, 2014
Filing Date:
February 17, 2014
Export Citation:
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Assignee:
NEC SOLUTION INNOVATORS LTD (?1368627, JP)
International Classes:
C12N15/09; C12Q1/68
Domestic Patent References:
WO2011016565A12011-02-10
WO2013005723A12013-01-10
Foreign References:
US5424186A1995-06-13
US5807522A1998-09-15
JPH10503841A1998-04-07
Other References:
CARSTEN TELLER ET AL.: "Aptamer-DNAzyme Hairpins for Amplified Biosensing", ANALYTICAL CHEMISTRY, vol. 81, no. 21, 1 November 2009 (2009-11-01), pages 9114 - 9119, XP055074517, DOI: doi:10.1021/ac901773b
DI LI ET AL.: "Amplified Analysis of Low- Molecular-Weight Substrates or Proteins by the Self-Assembly of DNAzyme-Aptamer Conjugates", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, pages 5804 - 5805, XP055148645, DOI: doi:10.1021/ja070180d
TELLER, ANAL. CHEM., vol. 81, 2009, pages 9114 - 9119
TRAVASCIO, CHEM. BIOL., vol. 5, 1998, pages 505 - 517
CHENG, BIOCHIMISTRY, vol. 48, 2009, pages 7817 - 7823
TAO, ANAL. CHEM., vol. 81, 2009, pages 2144 - 2149
Attorney, Agent or Firm:
TSUJIMARU Koichiro et al. (?6008813, JP)
Koichiro Tsujimaru (?6008813, JP)
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