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Title:
NUCLEIC ACID, FUSION PROTEIN, RECOMBINED CELL, AND ISOPRENE OR CYCLIC TERPENE PRODUCTION METHOD
Document Type and Number:
WIPO Patent Application WO/2016/129637
Kind Code:
A1
Abstract:
 Provided is a nucleic acid for coding a fusion protein that links a FKBP family protein with a first protein selected from the group consisting of isoprene synthases and cyclic terpene synthases. Also provided is a fusion protein coded by said nucleic acid. Further provided is a recombined cell having said nucleic acid and expressing said fusion protein. Yet further provided is a recombined cell having a first nucleic acid for coding said first protein and a second nucleic acid for coding a FKBP family protein, and expressing said first protein and said FKBP family protein. Syngas-utilizing bacteria and methane-utilizing bacteria can be used as the host cells.

Inventors:
FURUTANI MASAHIRO (JP)
KAWABATA KAZUFUMI (JP)
NISHIYAMA NORIHIDE (JP)
Application Number:
PCT/JP2016/053977
Publication Date:
August 18, 2016
Filing Date:
February 10, 2016
Export Citation:
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Assignee:
SEKISUI CHEMICAL CO LTD (JP)
International Classes:
C07K14/00; C12N15/09; C07K19/00; C12N1/21; C12N9/00; C12P5/00; C12P5/02; C12R1/145
Domestic Patent References:
WO2005063964A12005-07-14
WO2013166320A12013-11-07
WO2013179722A12013-12-05
Foreign References:
JP2005013067A2005-01-20
JP2006166845A2006-06-29
JP2014158489A2014-09-04
JP2014076042A2014-05-01
JP2015181428A2015-10-22
Other References:
IDENO, A. ET AL.: "Expression of foreign proteins in Escherichia coli by fusing with an archaeal FK506 binding protein", APPL. MICROBIOL. BIOTECHNOL., vol. 64, 2004, pages 99 - 105, XP002980658, ISSN: 0175-7598
See also references of EP 3257941A4
Attorney, Agent or Firm:
FUJITA Takashi et al. (JP)
Takashi Fujita (JP)
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