Title:
NUCLEIC ACID INCLUDING ARABINOSE-DEPENDENT GENE EXPRESSION CONTROL SEQUENCE
Document Type and Number:
WIPO Patent Application WO/2019/156152
Kind Code:
A1
Abstract:
The present disclosure relates to: a nucleic acid that includes a gene expression control sequence that can control the expression of a target gene in coryneform bacteria; bacteria into which the nucleic acid has been introduced; a method for using the bacteria to express the target gene; and a method for using the bacteria to produce a substance. At least a portion of the nucleic acid has an arbitrary promoter sequence and an AraR-binding sequence that is specified by a prescribed nucleotide sequence. When the nucleic acid has been introduced into coryneform bacteria, the promoter activity of the promoter sequence is suppressed so long as arabinose is not added and is induced when arabinose is added.
Inventors:
NAKANE SHUHEI (JP)
Application Number:
PCT/JP2019/004378
Publication Date:
August 15, 2019
Filing Date:
February 07, 2019
Export Citation:
Assignee:
GREEN EARTH INST CO LTD (JP)
International Classes:
C12N15/77; C12P21/02; C12N1/21; C12R1/15
Domestic Patent References:
| WO2008001597A1 | 2008-01-03 | |||
| WO2013069634A1 | 2013-05-16 |
Foreign References:
| JP2010535028A | 2010-11-18 |
Other References:
KUGE, TAKAYUKI: "Engineering of Corynebacterium glutamicum for arabinoxylan utilization", DOCTORAL DISSERTATION OF THE GRADUATE SCHOOL OF BIOLOGICAL SCIENCES OF NARA INSTITUTE OF SCIENCE AND TECHNOLOGY, March 2017 (2017-03-01), pages 1 - 120, Retrieved from the Internet [retrieved on 20190423]
KUGE, T. ET AL.: "The LacI-Type Transcriptional Regulator AraR Acts as an L-Arabinose-Responsive Repressor of L-Arabinose Utilization Genes in Corynebacterium glutamicum ATCC 31831", JOURNAL OF BACTERIOLOGY, vol. 196, no. 12, 4 April 2014 (2014-04-04) - June 2014 (2014-06-01), pages 2242 - 2254, XP055629741
KUGE, T. ET AL.: "AraR, an L-Arabinose-Responsive Transcriptional Regulator in Corynebacterium glutamicum ATCC 31831, Exerts Different Degrees of Repression Depending on the Location of Its Binding Sites within the Three Target Promoter Regions", JOURNAL OF BACTERIOLOGY, vol. 197, no. 24, 28 September 2015 (2015-09-28) - December 2015 (2015-12-01), pages 3788 - 3796, XP055629746
KUGE, TAKAYUKI ET AL.: "Arabinose response Control mechanism by transcription factor AraR in coryneform bacteria", PROGRAMS OF THE 2015 CONFERENCE OF JSBBA, 25 February 2015 (2015-02-25)
KUGE, T. ET AL.: "The LacI-Type Transcriptional Regulator AraR Acts as an L-Arabinose-Responsive Repressor of L-Arabinose Utilization Genes in Corynebacterium glutamicum ATCC 31831", JOURNAL OF BACTERIOLOGY, vol. 196, no. 12, 4 April 2014 (2014-04-04) - June 2014 (2014-06-01), pages 2242 - 2254, XP055629741
KUGE, T. ET AL.: "AraR, an L-Arabinose-Responsive Transcriptional Regulator in Corynebacterium glutamicum ATCC 31831, Exerts Different Degrees of Repression Depending on the Location of Its Binding Sites within the Three Target Promoter Regions", JOURNAL OF BACTERIOLOGY, vol. 197, no. 24, 28 September 2015 (2015-09-28) - December 2015 (2015-12-01), pages 3788 - 3796, XP055629746
KUGE, TAKAYUKI ET AL.: "Arabinose response Control mechanism by transcription factor AraR in coryneform bacteria", PROGRAMS OF THE 2015 CONFERENCE OF JSBBA, 25 February 2015 (2015-02-25)
Attorney, Agent or Firm:
YAGAMI Ayanobu (JP)
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