Title:
NUCLEIC ACID MOLECULE CAPABLE OF BINDING TO HMGB1, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2011/007876
Kind Code:
A1
Abstract:
Disclosed are a nucleic acid molecule which is capable of binding to HMGB1 protein, and a use of the nucleic acid molecule.
Specifically, a nucleic acid molecule having a dissociation constant for HMGB1 protein of 5 × 10-7 or less can be used as a nucleic acid molecule that is capable of binding to HMGB1 protein. Since the nucleic acid molecule capable of binding to HMGB1 protein is able to bind to HMGB1 protein, which is known as a cause of diseases such as cancers and inflammation, preventive and curative effects on such diseases can be attained by having the nucleic acid molecule bound to HMGB1 protein in vivo.
Inventors:
TAKENAKA HIROMI (JP)
AKITOMI JOU (JP)
KATOU SHINTAROU (JP)
TSUJI SHOTARO (JP)
OHTSU TAKASHI (JP)
WAGA IWAO (JP)
AKITOMI JOU (JP)
KATOU SHINTAROU (JP)
TSUJI SHOTARO (JP)
OHTSU TAKASHI (JP)
WAGA IWAO (JP)
Application Number:
PCT/JP2010/062104
Publication Date:
January 20, 2011
Filing Date:
July 16, 2010
Export Citation:
Assignee:
NEC SOFTWARE LTD (JP)
KANAGAWA PREFECTURAL HOSPITAL ORGANIZATION (JP)
TAKENAKA HIROMI (JP)
AKITOMI JOU (JP)
KATOU SHINTAROU (JP)
TSUJI SHOTARO (JP)
OHTSU TAKASHI (JP)
WAGA IWAO (JP)
KANAGAWA PREFECTURAL HOSPITAL ORGANIZATION (JP)
TAKENAKA HIROMI (JP)
AKITOMI JOU (JP)
KATOU SHINTAROU (JP)
TSUJI SHOTARO (JP)
OHTSU TAKASHI (JP)
WAGA IWAO (JP)
International Classes:
C12N15/09; A61K31/7088; A61K31/7105; A61P9/00; A61P29/00; A61P35/00; A61P35/04; C12Q1/68; G01N33/68
Domestic Patent References:
| WO2007076200A2 | 2007-07-05 |
Foreign References:
| JP3876325B1 | 2007-01-31 | |||
| JP2008520552A | 2008-06-19 |
Other References:
JAOUEN S. ET AL.: "Determinants of Specific Binding of HMGB1 Protein to Hemicatenated DNA Loops.", J. MOL. BIOL., vol. 353, no. 4, 4 November 2005 (2005-11-04), pages 822 - 837, XP005118793
WEBB M. ET AL.: "Structural requirements for cooperative binding of HMG1 to DNA minicircles.", J. MOL. BIOL., vol. 309, no. 1, 25 May 2001 (2001-05-25), pages 79 - 88, XP004464218
JAYASENA, S.: "Aptamers: an emerging class of molecules that rival antibodies in diagnostics.", CLIN. CHEM., vol. 45, no. 9, July 1999 (1999-07-01), pages 1628 - 1650, XP008067440
KEEFE, A.D. ET AL.: "SELEX with modified nucleotides.", CURR. OPIN. CHEM. BIOL., vol. 12, no. 4, August 2008 (2008-08-01), pages 448 - 456, XP024528065
OHTSU, T. ET AL.: "RNA aptamers against HMGB1 - the possibility to inhibit the cancer cell proliferation.", PROCEEDINGS OF THE JAPANESE CANCER ASSOCIATION, vol. 68TH, 31 August 2009 (2009-08-31), pages 494
See also references of EP 2455469A4
CALOGERO, S. ET AL., NAT. GENET., vol. 22, 1999, pages 276 - 280
BUSTIN, M., MOL. CELL BIOL., vol. 19, 1999, pages 523 - 5246
ANDERSSON, U. ET AL., J. LEUKOC. BIOL., vol. 72, 2002, pages 1084 - 1091
KLUNE, J. R. ET AL., MOL. MED., vol. 14, 2008, pages 476 - 484
ELLERMAN, J. E. ET AL., CLIN. CANCER RES., vol. 13, 2007, pages 2836 - 2848
LIU, K. ET AL., FASEB J., vol. 21, 2007, pages 3904 - 16
WEBB M. ET AL.: "Structural requirements for cooperative binding of HMG1 to DNA minicircles.", J. MOL. BIOL., vol. 309, no. 1, 25 May 2001 (2001-05-25), pages 79 - 88, XP004464218
JAYASENA, S.: "Aptamers: an emerging class of molecules that rival antibodies in diagnostics.", CLIN. CHEM., vol. 45, no. 9, July 1999 (1999-07-01), pages 1628 - 1650, XP008067440
KEEFE, A.D. ET AL.: "SELEX with modified nucleotides.", CURR. OPIN. CHEM. BIOL., vol. 12, no. 4, August 2008 (2008-08-01), pages 448 - 456, XP024528065
OHTSU, T. ET AL.: "RNA aptamers against HMGB1 - the possibility to inhibit the cancer cell proliferation.", PROCEEDINGS OF THE JAPANESE CANCER ASSOCIATION, vol. 68TH, 31 August 2009 (2009-08-31), pages 494
See also references of EP 2455469A4
CALOGERO, S. ET AL., NAT. GENET., vol. 22, 1999, pages 276 - 280
BUSTIN, M., MOL. CELL BIOL., vol. 19, 1999, pages 523 - 5246
ANDERSSON, U. ET AL., J. LEUKOC. BIOL., vol. 72, 2002, pages 1084 - 1091
KLUNE, J. R. ET AL., MOL. MED., vol. 14, 2008, pages 476 - 484
ELLERMAN, J. E. ET AL., CLIN. CANCER RES., vol. 13, 2007, pages 2836 - 2848
LIU, K. ET AL., FASEB J., vol. 21, 2007, pages 3904 - 16
Attorney, Agent or Firm:
TSUJIMARU Koichiro et al. (JP)
Koichiro Tsujimaru (JP)
Koichiro Tsujimaru (JP)
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