Title:
NUCLEIC ACID PRETREATMENT KIT, AND BASE SEQUENCE ANALYSIS METHOD
Document Type and Number:
WIPO Patent Application WO/2017/126521
Kind Code:
A1
Abstract:
A pretreatment kit includes a container (10) for manipulating particles, nucleic acid-trapping particles (70) capable of binding selectively with a nucleic acid, media (21, 22, 23) for water phase separation, multiple types of aqueous liquids (35, 31, 32, 38), and a nucleic acid for individual identification. The nucleic acid for individual identification is included in at least one of the multiple types of aqueous liquids or is bound to the surface of the nucleic acid-trapping particles; the base sequence of the nucleic acid for individual identification includes an identification sequence comprising a base sequence non-complementary to the nucleic acids included in the biological sample. The pretreatment kit is used to separate nucleic acids from biological samples including nucleic acids and contaminants.
Inventors:
KANAI MASAKI (JP)
JIKUYA HIROYUKI (JP)
OHASHI TETSUO (JP)
NAKAMURA SHIN (JP)
OHARA OSAMU (JP)
JIKUYA HIROYUKI (JP)
OHASHI TETSUO (JP)
NAKAMURA SHIN (JP)
OHARA OSAMU (JP)
Application Number:
PCT/JP2017/001468
Publication Date:
July 27, 2017
Filing Date:
January 18, 2017
Export Citation:
Assignee:
SHIMADZU CORP (JP)
KAZUSA DNA RES INST (JP)
KAZUSA DNA RES INST (JP)
International Classes:
C12M1/00; C12N15/09; C12Q1/68
Domestic Patent References:
| WO2012086243A1 | 2012-06-28 | |||
| WO2012176598A1 | 2012-12-27 |
Foreign References:
| JP2009060862A | 2009-03-26 | |||
| JP2013226506A | 2013-11-07 |
Attorney, Agent or Firm:
SHINTAKU, Masato et al. (JP)
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