Title:
NUCLEIC ACID FOR PRODUCING RAAV IN INSECT CELL, VP1 CAPSID PROTEIN MUTANT, AND USE
Document Type and Number:
WIPO Patent Application WO/2024/067153
Kind Code:
A1
Abstract:
Provided are a nucleic acid for producing rAAV in an insect cell, a VP1 capsid protein mutant, and use. The nucleic acid comprises nucleotide sequences encoding adeno-associated viruses VP1, VP2, and VP3 proteins. Compared with the capsid protein of wild-type AAV serotype 8, the amino acid sequence of the VP1 protein comprises a mutation at one or more sites among sites 84, 92, and 105, and the amino acid sequence of the VP2 protein is not mutated. The VP1 protein coding sequence is mutated, so that degradation of the VP1 protein in an insect cell can be reduced, and the expressed VP1, VP2, and VP3 can maintain a proper ratio to correctly assemble a capsid, thereby improving the yield and effectiveness of producing rAAV by using a baculovirus/insect cell expression system.
Inventors:
XIAO HE (CN)
HE XIAOBIN (CN)
HUANG GANG (CN)
HU YING (CN)
PAN XING (CN)
WANG MENGDIE (CN)
DU LIANG (CN)
HE XIAOBIN (CN)
HUANG GANG (CN)
HU YING (CN)
PAN XING (CN)
WANG MENGDIE (CN)
DU LIANG (CN)
Application Number:
PCT/CN2023/119002
Publication Date:
April 04, 2024
Filing Date:
September 15, 2023
Export Citation:
Assignee:
GENEVOYAGER WUHAN CO LTD (CN)
International Classes:
C12N15/35; C07K14/015; C12N5/10; C12N7/01; C12N15/65; C12N15/866
Attorney, Agent or Firm:
WUHAN HUAZHIYU INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
Download PDF: