Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
NUCLEIC ACID PURIFICATION METHOD, NUCLEIC ACID EXTRACTION METHOD, AND NUCLEIC ACID PURIFICATION KIT
Document Type and Number:
WIPO Patent Application WO/2013/038604
Kind Code:
A1
Abstract:
[Problem] To provide a nucleic acid purification method which involves simple operations and can purify nucleic acid at high efficiency in a short time. [Solution] A nucleic acid purification method including a procedure for adsorbing a substance, included in a sample containing nucleic acid, by an ion exchange resin (10), a cation exchange resin and an anion exchange resin being used for the ion exchange resin (10). A first cation exchange resin and a second cation exchange resin having a lesser molecular weight exclusion limit than that of the first cation exchange resin may be used for the cation exchange resin.

Inventors:
NAKAMURA TOMOHIKO (JP)
SAKAMOTO NAOHISA (JP)
YOTORIYAMA TASUKU (JP)
ITO KAZUMINE (JP)
Application Number:
PCT/JP2012/005384
Publication Date:
March 21, 2013
Filing Date:
August 28, 2012
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
SONY CORP (JP)
NAKAMURA TOMOHIKO (JP)
SAKAMOTO NAOHISA (JP)
YOTORIYAMA TASUKU (JP)
ITO KAZUMINE (JP)
International Classes:
C12N15/09; B01D15/04; B01J39/04; B01J41/04; C12M1/00; C12Q1/68; G01N30/88
Foreign References:
JP2007516425A2007-06-21
JP2005080555A2005-03-31
Other References:
HUBER, C.G. ET AL.: "On-line cation exchange for suppression of adduct formation in negative-ion electrospray mass spectrometry of nucleic acids", ANAL.CHEM., vol. 70, no. 24, 1998, pages 5288 - 5295, XP002953264
IKEGAMI, T. ET AL.: "Anion- and cation-exchange MicroHPLC utilizing poly(methacrylates)-coated monolithic silica capillary columns", ANAL.SCI., vol. 23, no. 1, 2007, pages 109 - 113, XP055143215
See also references of EP 2757156A4
Attorney, Agent or Firm:
OMORI, JUNICHI (JP)
Omori Junichi (JP)
Download PDF:
Claims: