Title:
NUCLEIC ACID SEQUENCING BY RAMAN MONITORING OF UPTAKE OF PRECURSORS DURING MOLECULAR REPLICATION
Document Type and Number:
WIPO Patent Application WO2003027307
Kind Code:
A3
Abstract:
The methods, compositions and apparatus disclosed herein are of use for nucleic acid sequence determination. The methods involve isolation of one or more nucleic acid template molecules and polymerization of a nascent complementary strand of nucleic acid, using a DNA or RNA polymerase or similar synthetic reagent. As the nascent strand is extended one nucleotide at a time, the disappearance of nucleotide precursors from solution is monitored by Raman spectroscopy or FRET. The nucleic acid sequence of the nascent strand, and the complementary sequence of the template strand, may be determined by tracking the order of incorporation of nucleotide precursors during the polymerization reaction. Certain embodiments concern apparatus comprising a reaction chamber and detection unit, of use in practicing the claimed methods. The methods, compositions and apparatus are of use in sequencing very long nucleic acid templates in a single sequencing reaction.
Inventors:
RAO VALLURI
NEUBAUER GABI
KIRCH STEVEN
YAMAKAWA MINEO
BERLIN ANDREW
NEUBAUER GABI
KIRCH STEVEN
YAMAKAWA MINEO
BERLIN ANDREW
Application Number:
PCT/US2002/028443
Publication Date:
November 06, 2003
Filing Date:
September 05, 2002
Export Citation:
Assignee:
INTEL CORP (US)
International Classes:
C12M1/00; C12M1/34; C12N15/00; C12N15/09; C12Q1/68; G01N21/65; G01N33/53; G01N33/566; B01L3/00; G01J3/44; (IPC1-7): C12Q1/68; C12P19/34; C12M1/34; G01J3/42; G01J3/427; C07H21/02; C07H21/04
Foreign References:
| US20020102595A1 | 2002-08-01 | |||
| US6017742A | 2000-01-25 | |||
| US6232075B1 | 2001-05-15 |
Other References:
COTTON T.M. ET AL.: "Application of surface-enhanced raman spectroscopy to biological systems", J. RAMAN SPECTROSCOPY, vol. 22, no. 12, December 1991 (1991-12-01), pages 729 - 742, XP000196566
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