Title:
NUCLEIC ACID SEQUENCING METHOD AND DEVICE BASED ON POROUS FILTER MEMBRANE
Document Type and Number:
WIPO Patent Application WO/2019/100910
Kind Code:
A1
Abstract:
Disclosed are a nucleic acid sequencing method and device based on a porous filter membrane. Specifically, the nucleic acid sequencing method specifically comprises: carrying out a nucleic acid sequencing reaction by hybridizing a nucleic acid probe fixed to a porous filter membrane with an amplified target gene or by amplifying a target gene using a nucleic acid primer fixed to a porous filter membrane and hybridizing the amplified target gene with a nucleic acid probe; and then detecting a reaction signal by detecting a change of any of a pH value, a pyrophosphoric acid concentration value, a light-emitting value or a fluorescence value generated in the nucleic acid sequencing reaction process. The present invention also relates to a nucleic acid sequencing device based on a porous filter membrane, comprising the following units: 1) a fluid control system; 2) a reactor; 3) a temperature control system; and 4) a sensor.
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Inventors:
RUAN SUFANG (CN)
Application Number:
PCT/CN2018/112416
Publication Date:
May 31, 2019
Filing Date:
October 29, 2018
Export Citation:
Assignee:
XIAMEN SNE BIOENGINEERING CO LTD (CN)
International Classes:
C12Q1/6869; C12Q1/6825; C12Q1/6834
Domestic Patent References:
| WO2003004690A2 | 2003-01-16 | |||
| WO2015132614A1 | 2015-09-11 |
Attorney, Agent or Firm:
BEIJING TINGLI PATENT AGENCY (ORDINARY PARTNERSHIP) (CN)
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