Title:
NUCLEIC ACID SYNTHESIS AND PURIFICATION DEVICE, USE THEREOF, AND NUCLEIC ACID SYNTHESIS AND PURIFICATION METHOD
Document Type and Number:
WIPO Patent Application WO/2020/118655
Kind Code:
A1
Abstract:
A nucleic acid synthesis and purification device, a use thereof, and a nucleic acid synthesis and purification method. The nucleic acid synthesis device comprises a solid phase carrier, wherein the solid phase carrier comprises a controlled pore glass (CPG); the CPG is an unmodified naked CPG, and a surface thereof has hydroxyl; the hydroxyl is used as a start point of nucleic acid synthesis, and the CPG is used for linking a phospholipid-protected nucleotide monomer or polymer by covalently bonding so as to perform nucleic acid synthesis. The nucleic acid synthesis device of the present invention can be used for not only synthesis of an oligonucleotide primer, but also for enzyme digestion and purification of a PCR product by using the oligonucleotide primer fixed on CPG, and has advantages of simple structure, small volume, light weight, high efficiency, low costs, and diversified functions.
Inventors:
FANG XIN (CN)
HUANG XIAOLUO (CN)
SHEN YUE (CN)
XU XUN (CN)
HUANG XIAOLUO (CN)
SHEN YUE (CN)
XU XUN (CN)
Application Number:
PCT/CN2018/121092
Publication Date:
June 18, 2020
Filing Date:
December 14, 2018
Export Citation:
Assignee:
BGI SHENZHEN (CN)
International Classes:
C12P19/34; C12M1/00; C12M1/40; C12N15/10
Foreign References:
| CN204151332U | 2015-02-11 | |||
| EP3284851A1 | 2018-02-21 | |||
| CN207483713U | 2018-06-12 | |||
| CN107383246A | 2017-11-24 |
Attorney, Agent or Firm:
DHC IP ATTORNEYS (CN)
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