Title:
NUCLEIC ACID/POLYSACCHARIDE COMPLEX
Document Type and Number:
WIPO Patent Application WO/2012/020795
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a highly stable nucleic acid/polysaccharide complex consisting of an siRNA and sizofiran.
The nucleic acid/polysaccharide complex is formed by adding polydeoxyadenine, at least a part of the phosphodiester bond moieties of which has been converted into phosphorothioate, to an siRNA and then forming a complex with sizofiran.
Inventors:
SAKURAI KAZUO (JP)
KOBAYASHI MASAKAZU (JP)
ANDO HIRONORI (JP)
HIGUCHI SADAHARU (JP)
KOBAYASHI MASAKAZU (JP)
ANDO HIRONORI (JP)
HIGUCHI SADAHARU (JP)
Application Number:
PCT/JP2011/068265
Publication Date:
February 16, 2012
Filing Date:
August 10, 2011
Export Citation:
Assignee:
NAPAJEN PHARMA INC (US)
SAKURAI KAZUO (JP)
KOBAYASHI MASAKAZU (JP)
ANDO HIRONORI (JP)
HIGUCHI SADAHARU (JP)
SAKURAI KAZUO (JP)
KOBAYASHI MASAKAZU (JP)
ANDO HIRONORI (JP)
HIGUCHI SADAHARU (JP)
International Classes:
C12N15/09; A61K31/787; A61K48/00; A61P43/00
Domestic Patent References:
| WO2009078470A1 | 2009-06-25 | |||
| WO2007058323A1 | 2007-05-24 |
Other References:
MIZU, MASAMI ET AL.: "Enhancement of the Antisense Effect of Polysaccharide- Polynucleotide Complexes by Preventing the Antisense Oligonucleotide from Binding to Proteins in the Culture Medium", BULL. CHEM. SOC. JPN., vol. 77, 2004, pages 1101 - 1110, XP008168537, DOI: doi:1O.1246/bcsj.77.1101
SUZUKI, MOTOHIKO ET AL.: "A novel allergen- specific therapy for allergy using CD40- silenced dendritic cells.", J. ALLERGY CLIN. IMMUNOL., vol. 125, no. 3, March 2010 (2010-03-01), pages 737 - 743, 743EL-743E6, XP026942672, DOI: doi:10.1016/j.jaci.2009.11.042
MOCHIZUKI, SHINICHI ET AL.: "Developement of nucleic acid delivery system for targeting antigen presenting cells.", POLYMER PREPRINTS, vol. 58, no. 2, 2009, JAPAN, pages 5018 - 5019, XP008170110
MOCHIZUKI, SHINICHI ET AL.: "Development of nucleic acid delivery system for targeting the antigen presenting cells", POLYMER PREPRINTS, vol. 58, no. 1, 2009, JAPAN, pages 1775
MOCHIZUKI, SHINICHI ET AL.: "A novel polysaccharide/polynucleotide complex and its application to bio-functional DNA delivery system.", POLYMER JOURNAL, vol. 41, no. 5, 2009, pages 343 - 353, XP055120059, DOI: doi:10.1295/polymj.PJ2008309
IKEDA, YOSHIHIKO ET AL.: "Blocking effect of anti-Dectin-1 antibodies on the anti-tumor activity of 1,3-beta-glucan and the binding of Dectin-1 to 1,3-beta-glucan.", BIOL. PHARM. BULL., vol. 30, no. 8, 2007, pages 1384 - 1389, XP008170111
SUZUKI, MOTOHIKO ET AL.: "A novel allergen- specific therapy for allergy using CD40- silenced dendritic cells.", J. ALLERGY CLIN. IMMUNOL., vol. 125, no. 3, March 2010 (2010-03-01), pages 737 - 743, 743EL-743E6, XP026942672, DOI: doi:10.1016/j.jaci.2009.11.042
MOCHIZUKI, SHINICHI ET AL.: "Developement of nucleic acid delivery system for targeting antigen presenting cells.", POLYMER PREPRINTS, vol. 58, no. 2, 2009, JAPAN, pages 5018 - 5019, XP008170110
MOCHIZUKI, SHINICHI ET AL.: "Development of nucleic acid delivery system for targeting the antigen presenting cells", POLYMER PREPRINTS, vol. 58, no. 1, 2009, JAPAN, pages 1775
MOCHIZUKI, SHINICHI ET AL.: "A novel polysaccharide/polynucleotide complex and its application to bio-functional DNA delivery system.", POLYMER JOURNAL, vol. 41, no. 5, 2009, pages 343 - 353, XP055120059, DOI: doi:10.1295/polymj.PJ2008309
IKEDA, YOSHIHIKO ET AL.: "Blocking effect of anti-Dectin-1 antibodies on the anti-tumor activity of 1,3-beta-glucan and the binding of Dectin-1 to 1,3-beta-glucan.", BIOL. PHARM. BULL., vol. 30, no. 8, 2007, pages 1384 - 1389, XP008170111
Attorney, Agent or Firm:
Yamada, Iichiro et al. (JP)
Yamada 威 1 郎 (JP)
Yamada 威 1 郎 (JP)
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