Title:
OFF-THE-SHELF HUMAN UMBILICAL CORD DERIVED MESENCHYMAL STEM CELL, PREPARATION METHOD AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/124185
Kind Code:
A1
Abstract:
The invention provides an off-the-shelf human umbilical cord derived mesenchymal stem cell, a preparation method thereof, and an application. The preparation method comprises the following steps: culturing an isolated umbilical cord tissue block to obtain a P0-generation cell; passaging the P0-generation cell twice to obtain a P2-generation cell and cryopreserving same; resuscitating the cryopreserved P2-generation cell, passaging twice to obtain a P4-generation cell and cryopreserving same; resuscitating the cryopreserved P4-generation cell, passaging once to obtain a P5-generation cell, and after sequential digestion, washing and resuspension of same, obtaining a human umbilical cord derived mesenchymal stem cell. Further, adopting a cryopreservation storage method, a human umbilical cord derived mesenchymal stem cell cryopreserved preparation is developed. The cryopreserved preparation is convenient to store, and after resuscitation, intravenous drug administration can be directly carried out, with no liquid change needed. Thus, the risk of possible pollution creation during medicine dispensing is avoided, facilitating clinical use. Further, the storage life of the cryopreserved preparation is long, and during the shelf life of same, multiple tests for preparation function and safety can be completed.
Inventors:
ZHANG YU (CN)
QI QI (CN)
ZHANG YUN (CN)
HOU RUIZHEN (CN)
WU WENJIE (CN)
ZHOU WANGYI (CN)
WANG LEI (CN)
QI QI (CN)
ZHANG YUN (CN)
HOU RUIZHEN (CN)
WU WENJIE (CN)
ZHOU WANGYI (CN)
WANG LEI (CN)
Application Number:
PCT/CN2022/116699
Publication Date:
July 06, 2023
Filing Date:
September 02, 2022
Export Citation:
Assignee:
WUHAN OPTICS VALLEY ZHONGYUAN PHARMACEUTICAL CO LTD (CN)
International Classes:
C12N5/0775; A01N1/02; A61K35/28; A61K35/51; A61P1/00; A61P1/16; A61P11/00; A61P29/00; A61P31/14
Domestic Patent References:
| WO2020173280A1 | 2020-09-03 |
Foreign References:
| CN114214276A | 2022-03-22 | |||
| CN113832548A | 2021-12-24 | |||
| CN112655702A | 2021-04-16 | |||
| CN104857022A | 2015-08-26 | |||
| CN111494420A | 2020-08-07 | |||
| CN104983742A | 2015-10-21 |
Attorney, Agent or Firm:
JEEKAI&PARTNERS (CN)
Download PDF:
Previous Patent: DRIVING DEVICE OF HYBRID VEHICLE
Next Patent: COMMUNICATION METHOD AND COMMUNICATION APPARATUS
Next Patent: COMMUNICATION METHOD AND COMMUNICATION APPARATUS