Title:
OLIGONUCLEOTIDE TRANSFECTION SCREENING METHOD
Document Type and Number:
WIPO Patent Application WO2002052046
Kind Code:
A3
Abstract:
Different-sequence peptoids, including lipid-and sterol-conjugated peptoids, are found to be effective in transfection of cells with oligonucleotides. Combinatorial libraries of such peptoids can be screened efficiently in a high-throughput format, and selected peptoids are identified post-screening.
Inventors:
ZUCKERMANN RONALD N (US)
REINHARD CHRISTOPH J (US)
JEFFERSON ANN B (US)
BEAUSOLEIL ERIC (US)
REINHARD CHRISTOPH J (US)
JEFFERSON ANN B (US)
BEAUSOLEIL ERIC (US)
Application Number:
PCT/US2001/049175
Publication Date:
February 12, 2004
Filing Date:
December 18, 2001
Export Citation:
Assignee:
CHIRON CORP (US)
ZUCKERMANN RONALD N (US)
REINHARD CHRISTOPH J (US)
JEFFERSON ANN B (US)
BEAUSOLEIL ERIC (US)
ZUCKERMANN RONALD N (US)
REINHARD CHRISTOPH J (US)
JEFFERSON ANN B (US)
BEAUSOLEIL ERIC (US)
International Classes:
G01N33/53; A61K51/04; C07K14/00; C12N15/87; C12N15/88; C12Q1/02; C12Q1/68; G01N37/00; (IPC1-7): C12Q1/68; G01N33/68; C07K14/00; C12N15/88
Domestic Patent References:
| WO1999008711A1 | 1999-02-25 | |||
| WO1998008092A1 | 1998-02-26 | |||
| WO1994002515A1 | 1994-02-03 | |||
| WO1992000091A1 | 1992-01-09 | |||
| WO1998006437A2 | 1998-02-19 | |||
| WO2001016306A2 | 2001-03-08 |
Other References:
MURPHY J E ET AL: "A combinatorial approach to the discovery of efficient cationic peptoid reagents for gene delivery.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. UNITED STATES 17 FEB 1998, vol. 95, no. 4, 17 February 1998 (1998-02-17), pages 1517 - 1522, XP002235261, ISSN: 0027-8424
LEBL M ET AL: "MULTIPLE RELEASE OF EQUIMOLAR AMOUNTS OF PEPTIDES FROM A POLYMERIC CARRIER USING ORTHOGONAL LINKAGE-CLEAVAGE CHEMISTRY", INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, MUNKSGAARD, COPENHAGEN, DK, vol. 41, no. 2, 1 February 1993 (1993-02-01), pages 201 - 203, XP000336695, ISSN: 0367-8377
LEBL M ET AL: "MULTIPLE RELEASE OF EQUIMOLAR AMOUNTS OF PEPTIDES FROM A POLYMERIC CARRIER USING ORTHOGONAL LINKAGE-CLEAVAGE CHEMISTRY", INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, MUNKSGAARD, COPENHAGEN, DK, vol. 41, no. 2, 1 February 1993 (1993-02-01), pages 201 - 203, XP000336695, ISSN: 0367-8377
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