Title:
OLIVETOL SYNTHETASE VARIANT AND ENGINEERED MICROORGANISM EXPRESSING SAME
Document Type and Number:
WIPO Patent Application WO/2023/035396
Kind Code:
A1
Abstract:
Provided are an olivetol synthetase variant and an engineered Escherichia coli expressing same for the production of olivetol and olivetolic acid. The engineered Escherichia coli is modified to express an olivetol synthetase variant which comprises one or more of the following mutants compared with a wild-type olivetol synthetase variant: I303T, I52L, S56A, H262M, and K263R. The engineered Escherichia coli achieves improved yields of olivetol and olivetolic acid.
Inventors:
DU DEYAO (CN)
WANG GAOYAN (CN)
ZHANG QIAN (CN)
ZONG ZHEN (CN)
LI MINGYUE (CN)
QIU YUEYUE (CN)
YIN JIN (CN)
ZHANG HAOQIAN (CN)
WANG GAOYAN (CN)
ZHANG QIAN (CN)
ZONG ZHEN (CN)
LI MINGYUE (CN)
QIU YUEYUE (CN)
YIN JIN (CN)
ZHANG HAOQIAN (CN)
Application Number:
PCT/CN2021/129656
Publication Date:
March 16, 2023
Filing Date:
November 09, 2021
Export Citation:
Assignee:
BEIJING BLUEPHA MICROBIOLOGY TECH CO LTD (CN)
SHENZHEN BLUEPHA BIOSCIENCES CO LTD (CN)
SHENZHEN BLUEPHA BIOSCIENCES CO LTD (CN)
International Classes:
C12N1/21; C12N15/53; C12N15/54; C12N15/60; C12N15/70; C12R1/19
Domestic Patent References:
| WO2021108617A1 | 2021-06-03 | |||
| WO2020214951A1 | 2020-10-22 | |||
| WO2020160289A1 | 2020-08-06 |
Foreign References:
| CN113502254A | 2021-10-15 | |||
| US20190352679A1 | 2019-11-21 | |||
| CN113366009A | 2021-09-07 | |||
| CN110914416A | 2020-03-24 |
Other References:
FLORES-SANCHEZ ISVETT J., LINTHORST HUUB J.M., VERPOORTE ROBERT: "In silicio expression analysis of PKS genes isolated from Cannabis sativa L.", GENETICS AND MOLECULAR BIOLOGY, SOCIEDADE BRASILEIRA DE GENETICA, RIBEIRAO PRETO, BR, vol. 33, no. 4, 31 December 2010 (2010-12-31), BR , pages 703 - 713, XP093043951, ISSN: 1415-4757, DOI: 10.1590/S1415-47572010005000088
TAURA, F. ; TANAKA, S. ; TAGUCHI, C. ; FUKAMIZU, T. ; TANAKA, H. ; SHOYAMA, Y. ; MORIMOTO, S.: "Characterization of olivetol synthase, a polyketide synthase putatively involved in cannabinoid biosynthetic pathway", FEBS LETTERS, ELSEVIER, AMSTERDAM., NL, vol. 583, no. 12, 18 June 2009 (2009-06-18), NL , pages 2061 - 2066, XP026185379, ISSN: 0014-5793, DOI: 10.1016/j.febslet.2009.05.024
DATABASE PROTEIN ANONYMOUS : "RecName: Full=3,5,7-trioxododecanoyl-CoA synthase; AltName: Full=Olivetol synthase; AltName: Full=Polyketide synthase-1; AltName: Full=Tetraketide synthase", XP093043954, retrieved from NCBI
TAURA, F. ; TANAKA, S. ; TAGUCHI, C. ; FUKAMIZU, T. ; TANAKA, H. ; SHOYAMA, Y. ; MORIMOTO, S.: "Characterization of olivetol synthase, a polyketide synthase putatively involved in cannabinoid biosynthetic pathway", FEBS LETTERS, ELSEVIER, AMSTERDAM., NL, vol. 583, no. 12, 18 June 2009 (2009-06-18), NL , pages 2061 - 2066, XP026185379, ISSN: 0014-5793, DOI: 10.1016/j.febslet.2009.05.024
DATABASE PROTEIN ANONYMOUS : "RecName: Full=3,5,7-trioxododecanoyl-CoA synthase; AltName: Full=Olivetol synthase; AltName: Full=Polyketide synthase-1; AltName: Full=Tetraketide synthase", XP093043954, retrieved from NCBI
Attorney, Agent or Firm:
KING & WOOD MALLESONS (CN)
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