Title:
ONCOLYTIC VIRUS FOR TREATING COLON CANCER BY USING NEWCASTLE DISEASE VIRUS SPECIFICALLY INFECTING COLON CANCER CELLS, AND COMPOSITION FOR TREATING COLON CANCER BY USING VIRUS
Document Type and Number:
WIPO Patent Application WO/2023/013812
Kind Code:
A1
Abstract:
The present invention relates to: an M2-LVP-K1 virus comprising a mutant sialic acid binding domain that is specific to colon cancer cells; and a composition for treating colon cancer, comprising the virus, and relates to a recombinant Newcastle disease virus in which a mutant sialic acid binding domain of the present invention, prepared using a directed evolution technology, is substituted for a normal sialic acid binding domain in a cell binding receptor HN protein so that specific infectivity for HCT116 cells is increased. The recombinant Newcastle disease virus M2-LVP-K1 having improved specific infectivity for colon cancer cells has a better HCT116 cell-killing effect than a conventional normal recombinant Newcastle disease virus, and it has been identified through in vivo experiments that the excellent effect of inhibiting cancer tissue growth is provided. The present invention relates to a therapeutic viral preparation in which a mutant recombinant Newcastle disease virus disclosed in the present study can induce a reduction in clinical symptoms, and partial or complete regression through colon cancer cell death or colon cancer tissue reduction.
Inventors:
JANG HYUN (KR)
JUNG BO KYOUNG (KR)
AN YONG HEE (KR)
JUNG BO KYOUNG (KR)
AN YONG HEE (KR)
Application Number:
PCT/KR2021/013148
Publication Date:
February 09, 2023
Filing Date:
September 27, 2021
Export Citation:
Assignee:
LIBENTECH CO LTD (KR)
International Classes:
C12N7/00; A61K35/768; A61P35/00; C07K14/005; C12N15/70; G01N33/68
Domestic Patent References:
| WO2019197275A1 | 2019-10-17 |
Foreign References:
| KR20080028277A | 2008-03-31 | |||
| KR20150127164A | 2015-11-16 | |||
| KR20200004408A | 2020-01-13 |
Other References:
TIRUMURUGAAN KRISHNASWAMY G., KAPGATE SUNIL, VINUPRIYA MANAVALAN K., VIJAYARANI KUMANAN, KUMANAN KATHAPERUMAL, ELANKUMARAN SUBBIAH: "Genotypic and Pathotypic Characterization of Newcastle Disease Viruses from India", PLOS ONE, vol. 6, no. 12, 1 January 2011 (2011-01-01), pages e28414, XP093031466, DOI: 10.1371/journal.pone.0028414
UDAYA S RANGASWAMY, WANG WEIJIA, CHENG XING, MCTAMNEY PATRICK, CARROLL DANIELLE, JIN HONG: "Newcastle Disease Virus Establishes Persistent Infection in Tumor Cells In Vitro : Contribution of the Cleavage Site of Fusion Protein and Second Sialic Acid Binding Site of Hemagglutinin-Neuraminidase", JOURNAL OF VIROLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, UNITED STATES, 15 August 2017 (2017-08-15), United States , XP055716021, Retrieved from the Internet DOI: 10.1128/JVI.00770-17
UDAYA S RANGASWAMY, WANG WEIJIA, CHENG XING, MCTAMNEY PATRICK, CARROLL DANIELLE, JIN HONG: "Newcastle Disease Virus Establishes Persistent Infection in Tumor Cells In Vitro : Contribution of the Cleavage Site of Fusion Protein and Second Sialic Acid Binding Site of Hemagglutinin-Neuraminidase", JOURNAL OF VIROLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, UNITED STATES, 15 August 2017 (2017-08-15), United States , XP055716021, Retrieved from the Internet
Attorney, Agent or Firm:
WIE, Byoung-Gap (KR)
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