Title:
ONE-STEP BIOMOLECULAR IMMOBILISATION PROCEDURE AND PRODUCTS THEREOF
Document Type and Number:
WIPO Patent Application WO/2014/056896
Kind Code:
A3
Abstract:
The invention relates to a method for covalent immobilisation of a biomolecule to a solid material and products arising therefrom, comprising providing a solid material with an activated surface, preferably by treatment with an oxidising agent, and subsequently contacting said material with a mixture of biomolecule to be immobilised and one or more silane components, preferably present in a pre-mixed binding solution. The immobilisation procedure is particularly useful for immunoassays, enzyme-linked immunosorbent assays (ELISA), surface plasmon resonance immunoassays, microarrays or microfluidic assays.
Inventors:
VASHIST SANDEEP KUMAR (DE)
ROTH GÜNTER (DE)
BURGER JÜRGEN (DE)
CZILWIK GREGOR (DE)
ROTH GÜNTER (DE)
BURGER JÜRGEN (DE)
CZILWIK GREGOR (DE)
Application Number:
PCT/EP2013/070919
Publication Date:
July 03, 2014
Filing Date:
October 08, 2013
Export Citation:
Assignee:
ALBERT LUDWIGS UNIVERSITÄT FREIBURG (DE)
HSG IMIT INST FÜR MIKRO UND INFORMATIONSTECHNOLOGIE (DE)
HSG IMIT INST FÜR MIKRO UND INFORMATIONSTECHNOLOGIE (DE)
International Classes:
B01L3/14
Domestic Patent References:
| WO2010044083A2 | 2010-04-22 |
Foreign References:
| US20050079486A1 | 2005-04-14 | |||
| EP1365249A1 | 2003-11-26 |
Other References:
CHANDRA KUMAR DIXIT ET AL: "Development of a High Sensitivity Rapid Sandwich ELISA Procedure and Its Comparison with the Conventional Approach", ANALYTICAL CHEMISTRY, vol. 82, no. 16, 15 August 2010 (2010-08-15), pages 7049 - 7052, XP055048836, ISSN: 0003-2700, DOI: 10.1021/ac101339q
SANDEEP KUMAR VASHIST ET AL: "Effect of antibody immobilization strategies on the analytical performance of a surface plasmon resonance-based immunoassay", THE ANALYST, vol. 136, no. 21, 1 January 2011 (2011-01-01), pages 4431, XP055048842, ISSN: 0003-2654, DOI: 10.1039/c1an15325k
DIXIT CHANDRA KUMAR ET AL: "Multisubstrate-compatible ELISA procedures for rapid and high-sensitivity immunoassays", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 6, no. 4, 1 April 2011 (2011-04-01), pages 439 - 445, XP009166031, ISSN: 1750-2799
DAN ZHENG ET AL: "Rapid and simple preparation of a reagentless glucose electrochemical biosensor", THE ANALYST, vol. 137, no. 16, 1 January 2012 (2012-01-01), pages 3800, XP055048865, ISSN: 0003-2654, DOI: 10.1039/c2an35128e
YANG LUO ET AL: "Sensitive and rapid quantification of C-reactive protein using quantum dot-labeled microplate immunoassay", JOURNAL OF TRANSLATIONAL MEDICINE, BIOMED CENTRAL, LONDON, GB, vol. 10, no. 1, 6 February 2012 (2012-02-06), pages 24, XP021118747, ISSN: 1479-5876, DOI: 10.1186/1479-5876-10-24
DAN ZHENG ET AL: "Mediatorless amperometric glucose biosensing using 3-aminopropyltriethoxysilane-functionalized graphene", TALANTA, vol. 99, 15 May 2012 (2012-05-15), pages 22 - 28, XP055093586, ISSN: 0039-9140, DOI: 10.1016/j.talanta.2012.05.014
SANDEEP KUMAR VASHIST: "Comparison of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Based Strategies to Crosslink Antibodies on Amine-Functionalized Platforms for Immunodiagnostic Applications", DIAGNOSTICS, vol. 2, no. 3, 27 August 2012 (2012-08-27), pages 23 - 33, XP055093581, DOI: 10.3390/diagnostics2030023
SANDEEP KUMAR VASHIST: "A sub-picogram sensitive rapid chemiluminescent immunoassay for the detection of human fetuin A", BIOSENSORS AND BIOELECTRONICS, vol. 40, no. 1, 1 February 2013 (2013-02-01), pages 297 - 302, XP055093588, ISSN: 0956-5663, DOI: 10.1016/j.bios.2012.07.067
S K VASHIST ET AL: "Rapid Immunodiagnostic Kits based on proprietary 1-step chemistry for covalent and leach-proof antibody immobilisation", MIKROSYSTEMTECHNIK - KONGRESS 2013, AACHEN, GERMANY, 14-16 OCT. 2013; POSTER 1.13, 15 October 2013 (2013-10-15), http://www.imtek.de/data/lehrstuehle/app/dokumente/conferences-pdf/conferences-2013/vashist-rapid-immunodiagnostic-kits, pages 384 - 387, XP055093601
SANDEEP KUMAR VASHIST ET AL: "Effect of antibody immobilization strategies on the analytical performance of a surface plasmon resonance-based immunoassay", THE ANALYST, vol. 136, no. 21, 1 January 2011 (2011-01-01), pages 4431, XP055048842, ISSN: 0003-2654, DOI: 10.1039/c1an15325k
DIXIT CHANDRA KUMAR ET AL: "Multisubstrate-compatible ELISA procedures for rapid and high-sensitivity immunoassays", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 6, no. 4, 1 April 2011 (2011-04-01), pages 439 - 445, XP009166031, ISSN: 1750-2799
DAN ZHENG ET AL: "Rapid and simple preparation of a reagentless glucose electrochemical biosensor", THE ANALYST, vol. 137, no. 16, 1 January 2012 (2012-01-01), pages 3800, XP055048865, ISSN: 0003-2654, DOI: 10.1039/c2an35128e
YANG LUO ET AL: "Sensitive and rapid quantification of C-reactive protein using quantum dot-labeled microplate immunoassay", JOURNAL OF TRANSLATIONAL MEDICINE, BIOMED CENTRAL, LONDON, GB, vol. 10, no. 1, 6 February 2012 (2012-02-06), pages 24, XP021118747, ISSN: 1479-5876, DOI: 10.1186/1479-5876-10-24
DAN ZHENG ET AL: "Mediatorless amperometric glucose biosensing using 3-aminopropyltriethoxysilane-functionalized graphene", TALANTA, vol. 99, 15 May 2012 (2012-05-15), pages 22 - 28, XP055093586, ISSN: 0039-9140, DOI: 10.1016/j.talanta.2012.05.014
SANDEEP KUMAR VASHIST: "Comparison of 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide Based Strategies to Crosslink Antibodies on Amine-Functionalized Platforms for Immunodiagnostic Applications", DIAGNOSTICS, vol. 2, no. 3, 27 August 2012 (2012-08-27), pages 23 - 33, XP055093581, DOI: 10.3390/diagnostics2030023
SANDEEP KUMAR VASHIST: "A sub-picogram sensitive rapid chemiluminescent immunoassay for the detection of human fetuin A", BIOSENSORS AND BIOELECTRONICS, vol. 40, no. 1, 1 February 2013 (2013-02-01), pages 297 - 302, XP055093588, ISSN: 0956-5663, DOI: 10.1016/j.bios.2012.07.067
S K VASHIST ET AL: "Rapid Immunodiagnostic Kits based on proprietary 1-step chemistry for covalent and leach-proof antibody immobilisation", MIKROSYSTEMTECHNIK - KONGRESS 2013, AACHEN, GERMANY, 14-16 OCT. 2013; POSTER 1.13, 15 October 2013 (2013-10-15), http://www.imtek.de/data/lehrstuehle/app/dokumente/conferences-pdf/conferences-2013/vashist-rapid-immunodiagnostic-kits, pages 384 - 387, XP055093601
Attorney, Agent or Firm:
BUCHANAN, Luke (Rechts- und PatentanwälteKurfürstendamm 54/55, Berlin, DE)
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