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Title:
OPTICAL MICROSCOPE DEVICE, MICROSCOPY METHOD, AND COMPUTER PROGRAM FOR MICROSCOPY USING SINGLE-LIGHT-EMITTING-PARTICLE DETECTION TECHNOLOGY
Document Type and Number:
WIPO Patent Application WO/2015/015951
Kind Code:
A1
Abstract:
This invention provides a microscopy technology that, using a scanning molecule-counting technique, makes it possible to detect light-emitting bodies or light-emitting particles that dynamically change in position in a thick sample. This optical microscopy technology detects light-emitting particles (LP) moving in a liquid by detecting light from said light-emitting particles (LP) via confocal microscope optics or multiphoton microscope optics. In a processing operation, light from a light-detection region (CV) is detected as said light-detection region (CV) is moved within each of a plurality of observation subregions (OsR) obtained by partitioning the observation region (ObR) of the microscope. This yields an individual signal corresponding to the light from each light-emitting particle (LP), and the position within the observation region (ObR) of the light-emitting particle (LP) corresponding to each detection signal is determined. The movement of the light-detection region (CV) within each observation subregion (OsR) may be performed a plurality of times in succession in the same direction and/or in two or more directions in each observation subregion (OsR).

Inventors:
YAMAGUCHI MITSUSHIRO (JP)
TANABE TETSUYA (JP)
Application Number:
PCT/JP2014/066541
Publication Date:
February 05, 2015
Filing Date:
June 23, 2014
Export Citation:
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Assignee:
OLYMPUS CORP (JP)
International Classes:
G01N21/64; G02B21/00
Domestic Patent References:
WO2013031439A12013-03-07
WO2013031365A12013-03-07
WO2013024650A12013-02-21
WO2012050011A12012-04-19
WO2011108369A12011-09-09
WO2011108370A12011-09-09
WO2011108371A12011-09-09
WO2012050011A12012-04-19
WO2012053355A12012-04-26
WO2013031439A12013-03-07
Foreign References:
JP2005017642A2005-01-20
JP2006162994A2006-06-22
Other References:
See also references of EP 3029505A4
Attorney, Agent or Firm:
AKASHI, Masaki et al. (JP)
Masatake Akashi (JP)
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