Title:
OPTICAL SWITCH PROTEIN THAT FUNCTIONS THROUGH IRRADIATION WITH LONG WAVELENGTH LIGHT
Document Type and Number:
WIPO Patent Application WO/2024/029527
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing an RpBphP1 mutant and an RpPpsR2 mutant that are modified so as to enable control of binding and unbinding therebetween with certainty through photoirradiation and blocking of near infrared light. More specifically, the present invention pertains to a protein set that comprises two proteins that bind with each other when being irradiated with near infrared light. The protein set is a set of an RpBphP1 mutant and the wild type RpPpsR2, a set of the wild type RpBphP1 and an RpPpsR2 mutant, or a set of the RpBphP1 mutant and the RpPpsR2 mutant. As compared to the binding of the wild type RpBphP1 and the wild type RpPpsR2, the binding among the protein set is weaker in a dark place and/or is stronger when being irradiated with near infrared light.
Inventors:
SATO MORITOSHI (JP)
NAKAJIMA TAKAHIRO (JP)
NAKAJIMA TAKAHIRO (JP)
Application Number:
PCT/JP2023/028106
Publication Date:
February 08, 2024
Filing Date:
August 01, 2023
Export Citation:
Assignee:
UNIV TOKYO (JP)
KANAGAWA INSTITUTE OF INDUSTRIAL SCIENCE AND TECH (JP)
KANAGAWA INSTITUTE OF INDUSTRIAL SCIENCE AND TECH (JP)
International Classes:
C07K14/195; C12N15/31
Domestic Patent References:
| WO2017059002A1 | 2017-04-06 |
Other References:
KABERNIUK ANDRII A, SHEMETOV ANTON A, VERKHUSHA VLADISLAV V: "A bacterial phytochrome-based optogenetic system controllable with near-infrared light", NATURE METHODS, NATURE PUBLISHING GROUP US, NEW YORK, vol. 13, no. 7, 1 July 2016 (2016-07-01), New York, pages 591 - 597, XP093135258, ISSN: 1548-7091, DOI: 10.1038/nmeth.3864
TARAS A REDCHUK, EVGENIYA S OMELINA, KONSTANTIN G CHERNOV, VLADISLAV V VERKHUSHA: "Near-infrared optogenetic pair for protein regulation and spectral multiplexing", NATURE CHEMICAL BIOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 13, no. 6, 1 June 2017 (2017-06-01), New York, pages 633 - 639, XP055696665, ISSN: 1552-4450, DOI: 10.1038/nchembio.2343
DATABASE Protein 24 July 2021 (2021-07-24), ANONYMOUS : "GAF domain-containing protein [Rhodopseudomonas palustris] ", XP093135261, retrieved from NCBI Database accession no. WP_119846077
TARAS A REDCHUK, EVGENIYA S OMELINA, KONSTANTIN G CHERNOV, VLADISLAV V VERKHUSHA: "Near-infrared optogenetic pair for protein regulation and spectral multiplexing", NATURE CHEMICAL BIOLOGY, NATURE PUBLISHING GROUP US, NEW YORK, vol. 13, no. 6, 1 June 2017 (2017-06-01), New York, pages 633 - 639, XP055696665, ISSN: 1552-4450, DOI: 10.1038/nchembio.2343
DATABASE Protein 24 July 2021 (2021-07-24), ANONYMOUS : "GAF domain-containing protein [Rhodopseudomonas palustris] ", XP093135261, retrieved from NCBI Database accession no. WP_119846077
Attorney, Agent or Firm:
OKUHARA Koji (JP)
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