Title:
OPTIMISED AND SIMPLIFIED CULTURE MEDIUM FOR THE PRODUCTION OF ETHANOL AND HYDROGEN FROM GLYCEROL, USING ESCHERICHIA COLI, IN ORDER TO BOOST BIOMASS PRODUCTIVITY
Document Type and Number:
WIPO Patent Application WO/2013/017710
Kind Code:
A3
Abstract:
The invention relates to an optimised and simplified culture medium for the production of ethanol and hydrogen from glycerol, using Escherichia coli. The invention consists of a specific culture medium for the process use for the biotransformation of glycerol into ethanol and hydrogen, using the microorganism E. coli MG1655. The aforementioned culture medium boosts the growth of the microorganism in order to obtain a concentration of E. coli, allowing higher ethanol production indices to be obtained compared to similar processes listed in the bibliography.
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Inventors:
GOMEZ MONTES DE OCA JOSE MANUEL (ES)
CANTERO MORENO DOMINGO (ES)
RAMIREZ MUNOZ MARTIN (ES)
CANTERO MORENO DOMINGO (ES)
RAMIREZ MUNOZ MARTIN (ES)
Application Number:
PCT/ES2012/000213
Publication Date:
March 28, 2013
Filing Date:
July 27, 2012
Export Citation:
Assignee:
UNIV CADIZ (ES)
GOMEZ MONTES DE OCA JOSE MANUEL (ES)
CANTERO MORENO DOMINGO (ES)
RAMIREZ MUNOZ MARTIN (ES)
COFRE CARVAJAL OCIEL (ES)
GOMEZ MONTES DE OCA JOSE MANUEL (ES)
CANTERO MORENO DOMINGO (ES)
RAMIREZ MUNOZ MARTIN (ES)
COFRE CARVAJAL OCIEL (ES)
International Classes:
C12N1/32
Domestic Patent References:
| WO2007115228A2 | 2007-10-11 |
Other References:
DHARMADI ,Y. ET AL.: "Anaerobic fermentation of glycerol by Escherichia coli: a new platform for metabolic engineering", BIOTECHNOLOGY AND BIOENGINEERING, vol. 94, no. 5, 2006, pages 821 - 819
SHAMS YAZDANI, S. ET AL.: "Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-products", METABOLIC ENGINEERING, vol. 10, no. 6, 2008, pages 340 - 351
GONZALEZ, R. ET AL.: "A new model for the anaerobic fermentation of glycerol in enteric bacteria: Trunk and auxiliary pathways in Escherichia coli", METABOLIC ENGINEERING, vol. 10, no. 5, 2008, pages 234 - 245
MURARKA, A. ET AL.: "Fermentative utilization of glycerol by Escherichia coli and its implications for the production of fuels and chemicals.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 74, no. 4, 2008, pages 1124 - 1135
TRINH, C.T. ET AL.: "Metabolic engineering of Escherichia coli for efficient conversion of glycerol to ethanol.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 75, no. 21, 2009, pages 6696 - 6705
SHAMS YAZDANI, S. ET AL.: "Engineering Escherichia coli for the efficient conversion of glycerol to ethanol and co-products", METABOLIC ENGINEERING, vol. 10, no. 6, 2008, pages 340 - 351
GONZALEZ, R. ET AL.: "A new model for the anaerobic fermentation of glycerol in enteric bacteria: Trunk and auxiliary pathways in Escherichia coli", METABOLIC ENGINEERING, vol. 10, no. 5, 2008, pages 234 - 245
MURARKA, A. ET AL.: "Fermentative utilization of glycerol by Escherichia coli and its implications for the production of fuels and chemicals.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 74, no. 4, 2008, pages 1124 - 1135
TRINH, C.T. ET AL.: "Metabolic engineering of Escherichia coli for efficient conversion of glycerol to ethanol.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 75, no. 21, 2009, pages 6696 - 6705
Attorney, Agent or Firm:
OTRI-UNIVERSIDAD DE CADIZ (C/Benito Pérez Goldós s/, Cádiz, ES)
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