Title:
OPTIMIZED METHOD FOR ISOLATING HUMAN INTESTINAL PROBIOTICS
Document Type and Number:
WIPO Patent Application WO/2017/066898
Kind Code:
A1
Abstract:
An optimized method for isolating human intestinal probiotics, comprising the following step: step 1, basal culture medium preparation: preparing a mucoprotein liquid culture medium and a mucoprotein solid culture medium; step 2, screening: collecting stool samples and inoculating the mucoprotein liquid culture medium with same, obtaining a bacteria solution by means of anaerobic culture, taking the bacteria solution as a template for carrying out a PCR experiment, selecting the bacteria solution in the tube having a positive result for a PCR reaction and inoculating the mucoprotein solid culture medium with the bacteria solution, obtaining bacterial colonies by means of anaerobic culture, inoculating a chocolate agar plate with the bacterial colonies on the mucoprotein solid culture medium , obtaining bacterial colonies by means of anaerobic culture, inoculating a Columbia blood agar plate with the bacterial colonies on the chocolate agar plate, obtaining bacterial colonies by means of purification and culture, and taking the bacterial colonies on the Columbia blood agar plate to perform PCR sequencing verification by using 16sRNA universal primers, thereby isolating human intestinal probiotics.
Inventors:
PENG YONGZHENG (CN)
YI JIANGFENG (CN)
GUO XIANFENG (CN)
YI JIANGFENG (CN)
GUO XIANFENG (CN)
Application Number:
PCT/CN2015/000809
Publication Date:
April 27, 2017
Filing Date:
November 23, 2015
Export Citation:
Assignee:
GUANGZHOU KANGZE MEDICAL TECH CO LTD (CN)
ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV (CN)
ZHUJIANG HOSPITAL SOUTHERN MEDICAL UNIV (CN)
International Classes:
C12N1/02
Foreign References:
| CN103525719A | 2014-01-22 | |||
| CN104232544A | 2014-12-24 | |||
| US20150290025A1 | 2015-10-15 | |||
| KR20140049399A | 2014-04-25 |
Other References:
AMAR, M.: "Isolation and Identification of Probiotics in the Intestines of the Longevous and the Research on the Longevous Mechanism", CHINA MASTER'S THESES FULL-TEXT DATABASE, 15 December 2012 (2012-12-15), ISSN: 1674-0246
WANG, BIN ET AL.: "Isolation and Identification of an Adhesive Probiotic Lactobacillus Strain from Human Gastrointestinal Tract", CHINESE JOURNAL OF BIOLOGICALS, vol. 21, no. 6, 30 June 2008 (2008-06-30), pages 463 - 467, ISSN: 1004-5503
KUTSCHER, M.A. ET AL.: "Isolation of Catechin-Converting Human Intestinal Bacteria", JOURNAL OF APPLIED MICROBIOLOGY, 31 December 2011 (2011-12-31), pages 165 - 175, XP055376483, ISSN: 1364-5072
WANG, BIN ET AL.: "Isolation and Identification of an Adhesive Probiotic Lactobacillus Strain from Human Gastrointestinal Tract", CHINESE JOURNAL OF BIOLOGICALS, vol. 21, no. 6, 30 June 2008 (2008-06-30), pages 463 - 467, ISSN: 1004-5503
KUTSCHER, M.A. ET AL.: "Isolation of Catechin-Converting Human Intestinal Bacteria", JOURNAL OF APPLIED MICROBIOLOGY, 31 December 2011 (2011-12-31), pages 165 - 175, XP055376483, ISSN: 1364-5072
Attorney, Agent or Firm:
GUANGZHOU KAIDONG INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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