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Title:
ORGANOID CULTURE CHIP AND ORGANOID CULTURE METHOD
Document Type and Number:
WIPO Patent Application WO/2023/020599
Kind Code:
A1
Abstract:
An organoid culture chip and culture method. The organoid culture chip comprises: a cell culture plate (1); and an organoid culture device (2) provided in the cell culture plate (1), wherein a culture medium reservoir (3) is formed between the organoid culture device (2) and the cell culture plate (1); and the organoid culture device (2) comprises: an organoid culture device body (21), a plurality of organoid culture chambers (22) provided in the organoid culture device body (21), and a plurality of side holes (23) formed in a side wall of each of two sides of the organoid culture device body (21), the organoid culture chamber (22) being in communication with the side hole (23) on a one-to-one basis to form a perfusion channel, and each of the organoid culture chambers (22) comprising a sample adding hole (221) formed in the top of same and a micropore (222) formed in the bottom of same, both the sample adding hole (221) and the micropore (222) being in communication with the bottom of the cell culture plate (1). By means of the organoid culture chip and culture method, the high-throughput and one-step dynamic perfusion culture of an organoid with a uniform morphology can be realized, and dynamic observation of the whole growth and development process of the organoid in situ can also be realized.

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Inventors:
CHEN PU (CN)
ZHAO WEN (CN)
CHEN TAO (CN)
Application Number:
PCT/CN2022/113449
Publication Date:
February 23, 2023
Filing Date:
August 19, 2022
Export Citation:
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Assignee:
UNIV WUHAN (CN)
International Classes:
C12M3/00; C12M1/00; C12M1/22; C12N5/0735; C12N5/095
Domestic Patent References:
WO2020242904A12020-12-03
Foreign References:
CN102858947A2013-01-02
JP2020010683A2020-01-23
CN207057848U2018-03-02
CN110072990A2019-07-30
CN113773959A2021-12-10
CN113388516A2021-09-14
CN1084887A1994-04-06
US5554536A1996-09-10
US20180127694A12018-05-10
Attorney, Agent or Firm:
WUHAN KEHAO INTELLECTUAL PROPERTY ATTORNEYS (SPECIAL GENERAL PARTNERSHIP) (CN)
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