Title:
OSTEOSARCOMA ORGANOID MODEL, CONSTRUCTION METHOD, AND USE
Document Type and Number:
WIPO Patent Application WO/2024/012073
Kind Code:
A1
Abstract:
Provided are an osteosarcoma organoid model and a construction method therefor. The osteosarcoma organoid model at least comprises a biological scaffold, osteosarcoma cells, and exosomes derived from mesenchymal stem cells, wherein the osteosarcoma cells and the exosomes are loaded on the biological scaffold, the biological scaffold is of a crisscrossed three-dimensional structure constructed by a hydrogel preparation, and the hydrogel preparation comprises a decellularized extracellular matrix and fibrin.
Inventors:
LU ZUYAN (CN)
DAI KERONG (CN)
DAI KERONG (CN)
Application Number:
PCT/CN2023/096652
Publication Date:
January 18, 2024
Filing Date:
May 26, 2023
Export Citation:
Assignee:
SHANGHAI 9TH PEOPLES HOSPITAL SHANGHAI JIAOTONG UNIV SCHOOL MEDICINE (CN)
International Classes:
C12N5/09; B01L3/00; C12M1/00; C12M3/00; C12N5/077; C12N5/0775; C12Q1/02
Domestic Patent References:
| WO2019122351A1 | 2019-06-27 | |||
| WO2022035275A1 | 2022-02-17 |
Foreign References:
| CN115651909A | 2023-01-31 | |||
| CN111808817A | 2020-10-23 | |||
| US20200330644A1 | 2020-10-22 |
Other References:
CONTESSI NEGRINI NICOLA, RICCI CLAUDIO, BONGIORNI FEDERICA, TROMBI LUISA, D’ALESSANDRO DELFO, DANTI SERENA, FARÈ SILVIA: "An Osteosarcoma Model by 3D Printed Polyurethane Scaffold and In Vitro Generated Bone Extracellular Matrix", CANCERS, CH, vol. 14, no. 8, CH , pages 2003, XP093128424, ISSN: 2072-6694, DOI: 10.3390/cancers14082003
YI, PING ET AL.: "Research Progress of Decellularized Matrix Hydrogels in 3-D Bioprinting", JOURNAL OF GUILIN MEDICAL UNIVERSITY, vol. 32, no. 4, 15 August 2019 (2019-08-15), pages 195 - 199, XP009551878, ISSN: 1008-2409, DOI: 10.19296/j.cnki.1008-2409.2019-04-055
YI, PING ET AL.: "Research Progress of Decellularized Matrix Hydrogels in 3-D Bioprinting", JOURNAL OF GUILIN MEDICAL UNIVERSITY, vol. 32, no. 4, 15 August 2019 (2019-08-15), pages 195 - 199, XP009551878, ISSN: 1008-2409, DOI: 10.19296/j.cnki.1008-2409.2019-04-055
Attorney, Agent or Firm:
J.Z.M.C. PATENT AND TRADEMARK LAW OFFICE (GENERAL PARTNERSHIP) (CN)
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