Title:
PHARMACEUTICAL COMPOSITION CONTAINING miR-140 AND METHOD FOR PRODUCING SAME
Document Type and Number:
WIPO Patent Application WO/2023/190271
Kind Code:
A1
Abstract:
[Problem] To provide: a pharmaceutical composition containing, as an active ingredient, exosomes containing a large amount of miR-140; and a method for producing the same. [Solution] A pharmaceutical composition containing exosomes derived from auricular chondrocytes, the exosomes containing miR-140; and a method for producing a pharmaceutical composition, the method including an exosome acquisition step for obtaining exosomes from auricular chondrocytes. The exosome acquisition step includes an auricular chondrocyte culture step for culturing auricular chondrocytes to obtain cultured chondrocytes, and an exosome collection step for collecting exosomes from the cultured chondrocytes cultured at the auricular chondrocyte culture step. At the auricular chondrocyte culture step, the medium in which the auricular chondrocytes are cultured contains estrogen.
Inventors:
YANAGA HIROKO (JP)
NISHINA HIROMICHI (JP)
YANAGA KATSU (JP)
NISHINA HIROMICHI (JP)
YANAGA KATSU (JP)
Application Number:
PCT/JP2023/012062
Publication Date:
October 05, 2023
Filing Date:
March 26, 2023
Export Citation:
Assignee:
REGENESIS SCIENCE CO LTD (JP)
International Classes:
A61K35/32; A61K9/08; A61K31/7105; A61K48/00; A61P19/02; A61P35/00; C12N5/077; C12N5/10; C12N15/113; C12N15/88
Domestic Patent References:
| WO2016076102A1 | 2016-05-19 |
Foreign References:
| KR20200018845A | 2020-02-21 | |||
| JP2022501034A | 2022-01-06 | |||
| KR20180092348A | 2018-08-20 |
Other References:
CHEN YAHONG, XUE KE, ZHANG XIAODIE, ZHENG ZHIWEI, LIU KAI: "Exosomes derived from mature chondrocytes facilitate subcutaneous stable ectopic chondrogenesis of cartilage progenitor cells", STEM CELL RESEARCH & THERAPY, vol. 9, no. 1, 1 December 2018 (2018-12-01), pages 318, XP093096169, DOI: 10.1186/s13287-018-1047-2
TAKADA T., T. KATO, T. NAKASA, H. ASAHARA, M. LOTZ, M. OCHI, S. MIYAK: "49. Exosomes containing micrornas function in a novel communication pathway among joint tissue cells. ", OSTEOARTHRITIS AND CARTILAGE, vol. 19, no. S1, 1 January 2011 (2011-01-01), pages S28, XP093096184
TAKADA T., T. KATO, T. NAKASA, H. ASAHARA, M. LOTZ, M. OCHI, S. MIYAK: "49. Exosomes containing micrornas function in a novel communication pathway among joint tissue cells. ", OSTEOARTHRITIS AND CARTILAGE, vol. 19, no. S1, 1 January 2011 (2011-01-01), pages S28, XP093096184
Attorney, Agent or Firm:
HIROSE Takayuki (JP)
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