Title:
PROCESS FOR PRODUCING PYRIDOXINE-5’-&agr -GLUCOSIDE
Document Type and Number:
WIPO Patent Application WO/2003/062442
Kind Code:
A1
Abstract:
A process for producing pyridoxine-5’-&agr -glucoside by glucosylating pyridoxine at the 5’-position which involves the step of treating pyridoxine or its salt with microbial cells capable of selectively glucosylating pyridoxine at the 5’-position to thereby form pyridoxine-5’-&agr -glucoside, a culture thereof or processed cells thereof; and a process for producing pyridoxine-5’-&agr -glucoside by treating pyridoxine or its salt with a glucosylating enzyme, characterized in that the treatment is carried out in the presence of boric acid and/or a boric acid salt to thereby enhance the selectivity of the glucosylation at the 5’-position. According to these methods, pyridoxine-5’-&agr -glucoside can be easily and effectively produced from pyridoxine or its salt.
Inventors:
WADA KOICHI (JP)
SAKAMOTO KEIJI (JP)
ASANO YASUHISA (JP)
SAKAMOTO KEIJI (JP)
ASANO YASUHISA (JP)
Application Number:
PCT/JP2003/000543
Publication Date:
July 31, 2003
Filing Date:
January 22, 2003
Export Citation:
Assignee:
DAIICHI FINE CHEM CO LTD (JP)
WADA KOICHI (JP)
SAKAMOTO KEIJI (JP)
ASANO YASUHISA (JP)
WADA KOICHI (JP)
SAKAMOTO KEIJI (JP)
ASANO YASUHISA (JP)
International Classes:
C12P17/12; C12P19/58; (IPC1-7): C12P19/58
Foreign References:
| JP2001238673A | 2001-09-04 | |||
| JPS4633198B1 | 1971-09-28 | |||
| JPS4918230B1 | 1974-05-08 |
Other References:
YASUHISA ASANO ET AL.: "Biseibutsu ni yoru pyridoxine no ichi sentakuteki glycosyl-ka", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY AND AGROCHEMISTRY TAIKAI KOEN YOSHISHU (3-5FP20), 5 March 2002 (2002-03-05), pages 198, XP002966892
SUZUKI Y. ET AL.: "Enzymatic preparation of pyridoxine 4'-5'-alpha D-glucosides", METHODS IN ENZYMOLOGY, vol. 280, 1997, pages 66 - 71, XP002965792
HOSOKAWA T. ET AL.: "Immobilization of CGTase from bacillus macerans and glycosidation of pyrodixine with its immobilized enzyme", SEITO GIJUTSU KENKYU KAISHI, vol. 48, 2001, pages 55 - 62
SUZUKI Y. ET AL.: "Enzymatic preparation of pyridoxine 4'-5'-alpha D-glucosides", METHODS IN ENZYMOLOGY, vol. 280, 1997, pages 66 - 71, XP002965792
HOSOKAWA T. ET AL.: "Immobilization of CGTase from bacillus macerans and glycosidation of pyrodixine with its immobilized enzyme", SEITO GIJUTSU KENKYU KAISHI, vol. 48, 2001, pages 55 - 62
Attorney, Agent or Firm:
SIKS & CO. (Kyobashi-Nisshoku Bldg. 8-7, Kyobashi 1-chom, Chuo-ku Tokyo, JP)
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