Title:
PAIRED END SEQUENCING
Document Type and Number:
WIPO Patent Application WO2007145612
Kind Code:
A8
Abstract:
The present invention provides for a method of preparing a target nucleic acid fragments to produce a smaller nucleic acid which comprises the two ends of the target nucleic acid. Specifically, the invention provides cloning and DNA manipulation strategies to isolate the two ends of a large target nucleic acid into a single small DNA construct for rapid cloning, sequencing, or amplification.
Inventors:
BERKA JAN (US)
CHEN ZHOUTAO (US)
EGHOLM MICHAEL (US)
GODWIN BRIAN C (US)
HUTCHISON STEPHEN K (US)
LEAMON JOHN H (US)
SARKIS GARY J (US)
SIMONS JAN F (US)
CHEN ZHOUTAO (US)
EGHOLM MICHAEL (US)
GODWIN BRIAN C (US)
HUTCHISON STEPHEN K (US)
LEAMON JOHN H (US)
SARKIS GARY J (US)
SIMONS JAN F (US)
Application Number:
PCT/US2006/022206
Publication Date:
February 28, 2008
Filing Date:
June 06, 2006
Export Citation:
Assignee:
454 LIFE SCIENCES CORP (US)
BERKA JAN (US)
CHEN ZHOUTAO (US)
EGHOLM MICHAEL (US)
GODWIN BRIAN C (US)
HUTCHISON STEPHEN K (US)
LEAMON JOHN H (US)
SARKIS GARY J (US)
SIMONS JAN F (US)
BERKA JAN (US)
CHEN ZHOUTAO (US)
EGHOLM MICHAEL (US)
GODWIN BRIAN C (US)
HUTCHISON STEPHEN K (US)
LEAMON JOHN H (US)
SARKIS GARY J (US)
SIMONS JAN F (US)
International Classes:
C12N15/10; C12Q1/68
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