Title:
PANCREATIC ISLET SEPARATION METHOD, AND PROTECTIVE SOLUTION FOR PROTECTING PANCREATIC ISLET TISSUE
Document Type and Number:
WIPO Patent Application WO/2012/008496
Kind Code:
A1
Abstract:
The disclosed pancreatic islet separation method comprises: an injection step wherein a protective solution is injected into the pancreatic duct of an extracted pancreas; a preservation step wherein the pancreas is immersed into an immersion fluid and preserved; a digestion step wherein the pancreas is broken down and pancreatic tissue is obtained; and a purification step wherein the pancreatic tissue is immersed in a purification solution and pancreatic islets are obtained. The digestion step comprises: an enzyme injection step wherein an enzyme solution containing digestion enzymes is injected into the pancreas; a digestion initiation step wherein the digestion enzymes are activated; a digestion termination step wherein the digestion enzymes are de-activated; and a collection step wherein the broken down pancreatic tissue is collected. The pancreatic islet separation method is characterised in that, by adding a neutrophil elastase inhibitor to the system before the digestion initiation step, the neutrophil elastase inhibitor is present inside the pancreas at the point when the digestion initiation step is commenced. Also, by using a protective solution which can be used in the above method, pancreatic islets having a size and shape suitable for transplanting can be obtained in high yields.
Inventors:
TANEMURA MASAHIRO
SAWA YOSHIKI
MYOUI AKIRA
ITO TOSHINORI
MORI MASAKI
DOKI YUICHIRO
SAWA YOSHIKI
MYOUI AKIRA
ITO TOSHINORI
MORI MASAKI
DOKI YUICHIRO
Application Number:
PCT/JP2011/065997
Publication Date:
January 19, 2012
Filing Date:
July 13, 2011
Export Citation:
Assignee:
OTSUKA PHARMA CO LTD (JP)
TANEMURA MASAHIRO
SAWA YOSHIKI
MYOUI AKIRA
ITO TOSHINORI
MORI MASAKI
DOKI YUICHIRO
TANEMURA MASAHIRO
SAWA YOSHIKI
MYOUI AKIRA
ITO TOSHINORI
MORI MASAKI
DOKI YUICHIRO
International Classes:
C12N5/071; C12N9/99; C12N9/50
Domestic Patent References:
| WO2006068226A1 | 2006-06-29 | |||
| WO2006068226A1 | 2006-06-29 |
Foreign References:
| JPS62265981A | 1987-11-18 |
Other References:
KUNIHIRO MASUKO ET AL.: "Sequential change of several parameters and the role of ulinastatin administration in acute pancreatitis.", THE JAPANESE JOURNAL OF ACUTE MEDICINE, vol. 19, no. 13, 1995, pages 1945 - 1952, XP009174239
OTA, M. ET AL.: "The effect of sivelestat sodium hydrate on ischemic reperfusion injury of the segmental pancreatic autotransplantation in the swine.", AMERICAN JOURNAL OF TRANSPLANTATION, vol. 7, no. SUPP.2, May 2007 (2007-05-01), pages 510 - 510, XP009174226
MIYAMOTO M. ET AL.: "Improved large-scale isolation of breeder porcine islets: Possibility of harvesting from nonheart-beating donor.", CELL TRANSPLANTATION, vol. 7, no. 4, 1998, pages 397 - 402, XP003000409
YAMANO M. ET AL.: "Protective effect of the combined treatment of pancreatic and neutrophil elastase inhibitors on acute pancreatitis elicited by lipopolysaccharide in rats given intraductal injection of taurocholate plus trypsin.", NAUNYN-SCHMIEDEBERG'S ARCH PHARMACOL, vol. 357, no. 5, 1998, pages 558 - 564, XP055072970
YOSHIAKI OMURA ET AL.: "Significant improvement of islet yield and viability in islet isolation with modified ET-Kyoto solution (ET-Kyoto/ neutrophil elastase inhibitor)", JOURNAL OF JAPAN SURGICAL SOCIETY, vol. 112, no. 1-2, 25 May 2011 (2011-05-25), pages 750, XP008165852
TRANSPLANTATION PROCEEDINGS, vol. 35, no. 7, 2003, pages 2455 - 7
See also references of EP 2594634A4
OTA, M. ET AL.: "The effect of sivelestat sodium hydrate on ischemic reperfusion injury of the segmental pancreatic autotransplantation in the swine.", AMERICAN JOURNAL OF TRANSPLANTATION, vol. 7, no. SUPP.2, May 2007 (2007-05-01), pages 510 - 510, XP009174226
MIYAMOTO M. ET AL.: "Improved large-scale isolation of breeder porcine islets: Possibility of harvesting from nonheart-beating donor.", CELL TRANSPLANTATION, vol. 7, no. 4, 1998, pages 397 - 402, XP003000409
YAMANO M. ET AL.: "Protective effect of the combined treatment of pancreatic and neutrophil elastase inhibitors on acute pancreatitis elicited by lipopolysaccharide in rats given intraductal injection of taurocholate plus trypsin.", NAUNYN-SCHMIEDEBERG'S ARCH PHARMACOL, vol. 357, no. 5, 1998, pages 558 - 564, XP055072970
YOSHIAKI OMURA ET AL.: "Significant improvement of islet yield and viability in islet isolation with modified ET-Kyoto solution (ET-Kyoto/ neutrophil elastase inhibitor)", JOURNAL OF JAPAN SURGICAL SOCIETY, vol. 112, no. 1-2, 25 May 2011 (2011-05-25), pages 750, XP008165852
TRANSPLANTATION PROCEEDINGS, vol. 35, no. 7, 2003, pages 2455 - 7
See also references of EP 2594634A4
Attorney, Agent or Firm:
OGURI Shohei et al. (JP)
Shohei Oguri (JP)
Shohei Oguri (JP)
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