Title:
PAPER-BASED ANALYTICAL MICROFLUIDIC CHIP AND DEVICE, HAVING EMBOSSED & DEBOSSED PROCESSING CHANNEL, FOR NUCLEIC ACID DIAGNOSIS
Document Type and Number:
WIPO Patent Application WO/2018/226193
Kind Code:
A3
Abstract:
The present invention is related to a method for providing sample preparation, nucleic acid extraction and diagnosis of cancer and infectious diseases from nucleic acids in samples like blood, tissue, saliva, urine by means of diagnosis device (10) comprising a measurement part (12) and a paper-based chip (11) to be inserted into said device, where said method is performed. The extracted nucleic acid is bound to metal nanoparticles and magnetic nanoparticles and detected by electrochemistry using an electrode, for example a graphene modified pencil lead electrode.
Inventors:
KARAKAYA MELIKE (TR)
KARAKAYA SAIT FATIH (TR)
KARAKAYA SAIT FATIH (TR)
Application Number:
PCT/TR2018/050126
Publication Date:
April 18, 2019
Filing Date:
March 28, 2018
Export Citation:
Assignee:
KARAKAYA MELIKE (TR)
International Classes:
C12Q1/6806; B01L3/00; C12Q1/6825
Foreign References:
| US20140335527A1 | 2014-11-13 | |||
| US20160327510A1 | 2016-11-10 |
Other References:
KOKKINOS CHRISTOS ET AL: "Emerging trends in biosensing using stripping voltammetric detection of metal-containing nanolabels - A review", ANALYTICA CHIMICA ACTA, ELSEVIER, AMSTERDAM, NL, vol. 961, 24 January 2017 (2017-01-24), pages 12 - 32, XP029927210, ISSN: 0003-2670, DOI: 10.1016/J.ACA.2017.01.016
JARUWAN METTAKOONPITAK ET AL: "Electrochemistry on Paper-based Analytical Devices: A Review", ELECTROANALYSIS, vol. 28, no. 7, 1 July 2016 (2016-07-01), US, pages 1420 - 1436, XP055569055, ISSN: 1040-0397, DOI: 10.1002/elan.201501143
NATALIA M. RODRIGUEZ ET AL: "A fully integrated paperfluidic molecular diagnostic chip for the extraction, amplification, and detection of nucleic acids from clinical samples", LAB ON A CHIP, vol. 16, no. 4, 1 January 2016 (2016-01-01), pages 753 - 763, XP055567189, ISSN: 1473-0197, DOI: 10.1039/C5LC01392E
XIANG LI ET AL: "Detection of Hepatitis B Virus DNA with a Paper Electrochemical Sensor", ANALYTICAL CHEMISTRY, vol. 87, no. 17, 10 August 2015 (2015-08-10), US, pages 9009 - 9015, XP055569046, ISSN: 0003-2700, DOI: 10.1021/acs.analchem.5b02210
JOHN T. CONNELLY ET AL: ""Paper Machine" for Molecular Diagnostics", ANALYTICAL CHEMISTRY, vol. 87, no. 15, 4 August 2015 (2015-08-04), US, pages 7595 - 7601, XP055330797, ISSN: 0003-2700, DOI: 10.1021/acs.analchem.5b00411
MELIKE KARAKAYA: "Analytical Molecular Diagnosis of Cervical Cancer via Paper Microfluidic Chip", PROCEEDINGS, vol. 2, no. 25, 20 December 2018 (2018-12-20), pages 1556, XP055567129, DOI: 10.3390/proceedings2251556
JARUWAN METTAKOONPITAK ET AL: "Electrochemistry on Paper-based Analytical Devices: A Review", ELECTROANALYSIS, vol. 28, no. 7, 1 July 2016 (2016-07-01), US, pages 1420 - 1436, XP055569055, ISSN: 1040-0397, DOI: 10.1002/elan.201501143
NATALIA M. RODRIGUEZ ET AL: "A fully integrated paperfluidic molecular diagnostic chip for the extraction, amplification, and detection of nucleic acids from clinical samples", LAB ON A CHIP, vol. 16, no. 4, 1 January 2016 (2016-01-01), pages 753 - 763, XP055567189, ISSN: 1473-0197, DOI: 10.1039/C5LC01392E
XIANG LI ET AL: "Detection of Hepatitis B Virus DNA with a Paper Electrochemical Sensor", ANALYTICAL CHEMISTRY, vol. 87, no. 17, 10 August 2015 (2015-08-10), US, pages 9009 - 9015, XP055569046, ISSN: 0003-2700, DOI: 10.1021/acs.analchem.5b02210
JOHN T. CONNELLY ET AL: ""Paper Machine" for Molecular Diagnostics", ANALYTICAL CHEMISTRY, vol. 87, no. 15, 4 August 2015 (2015-08-04), US, pages 7595 - 7601, XP055330797, ISSN: 0003-2700, DOI: 10.1021/acs.analchem.5b00411
MELIKE KARAKAYA: "Analytical Molecular Diagnosis of Cervical Cancer via Paper Microfluidic Chip", PROCEEDINGS, vol. 2, no. 25, 20 December 2018 (2018-12-20), pages 1556, XP055567129, DOI: 10.3390/proceedings2251556
Attorney, Agent or Firm:
KAYA, Erdem (TR)
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