Title:
PARASITIC PHYTOPHTHORA-DERIVED Ω-3 FATTY ACID DESATURASE FOR SYNTHESIZING POLYUNSATURATED FATTY ACIDS, CARRIER CONTAINING FATTY ACID DESATURASE, RECOMBINANT MICROORGANISMS, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/097218
Kind Code:
A1
Abstract:
A recombinant nucleic acid sequence, as shown in SEQ ID NO.3, of parasitic phytophthora-encoded ω-3 fatty acid desaturase, a carrier containing ω-3 fatty acid desaturase, recombinant saccharomyces cerevisiae and recombinant mortierella alpina which contain the carrier, and a construction method of a recombinant engineering strain. According to the use of the recombinant saccharomyces cerevisiae in the biosynthesis of polyunsaturated fatty acids, the recombinant saccharomyces cerevisiae can catalyze C20:4 Δ5,8,11,14 into C20:5 Δ5,8,11,14,17 at room temperature, and the catalytic efficiency reaches 65%. The content of EPA in a fermentation product of the recombinant mortierella alpina reaches 31.5% of the total amount of fatty acids.
Inventors:
CHEN HAIQIN (CN)
CHEN YONGQUAN (CN)
CHEN WEI (CN)
MEI TIANTIAN (CN)
GU ZHENNAN (CN)
ZHANG HAO (CN)
CHEN YONGQUAN (CN)
CHEN WEI (CN)
MEI TIANTIAN (CN)
GU ZHENNAN (CN)
ZHANG HAO (CN)
Application Number:
PCT/CN2016/108983
Publication Date:
June 15, 2017
Filing Date:
December 08, 2016
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N1/19; C12N15/53; C12N9/02; C12N15/81; C12P7/64; C12R1/865
Foreign References:
| CN105368851A | 2016-03-02 | |||
| CN105647822A | 2016-06-08 | |||
| CN101646766A | 2010-02-10 | |||
| CN103820335A | 2014-05-28 | |||
| CN102776213A | 2012-11-14 |
Other References:
XUE, ZHIXIONG: "Identification and characterization of new A-17 fatty acid desaturases", APPL. MICROBIOL. BIOTECHNOL., vol. 97, no. 5, 27 May 2012 (2012-05-27), pages 1973 - 1985, XP055273434
DATABASE Nucleotide 27 August 2014 (2014-08-27), RUSS, C.: "Phytophthora parasitica INRA-310 hypothetical protein mRNA", XP055599161, Database accession no. XM_008906963.1
DATABASE Nucleotide 27 August 2014 (2014-08-27), RUSS, C.: "Phytophthora parasitica INRA-310 hypothetical protein mRNA", XP055599161, Database accession no. XM_008906963.1
Attorney, Agent or Firm:
BEIJING JUNZHI INTELLECTUAL PROPERTY AGENCY (G. P.) (CN)
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