Title:
PARTIAL ENZYMATIC HYDROLYSIS OF TRIACYLGLYCEROLS
Document Type and Number:
WIPO Patent Application WO/2017/115322
Kind Code:
A3
Abstract:
Disclosed herein are host cells expressing a polypeptide, wherein the polypeptide hydrolyzes an ester linkage of a triacylglycerol in an oil comprising at least one long-chain polyunsaturated fatty acid, methods for using such host cells, and processes for production of a lipase using such host cells.
Inventors:
MUGFORD PAUL (CA)
MUELLER MONIKA (DE)
SCHURMANN MARTIN (DE)
MUELLER MONIKA (DE)
SCHURMANN MARTIN (DE)
Application Number:
PCT/IB2016/058086
Publication Date:
August 10, 2017
Filing Date:
December 29, 2016
Export Citation:
Assignee:
DSM IP ASSETS BV (NL)
International Classes:
C12P7/64; C11B3/00; C12N9/20
Domestic Patent References:
| WO2015087833A1 | 2015-06-18 | |||
| WO2013043641A1 | 2013-03-28 |
Foreign References:
| CN104726477A | 2015-06-24 |
Other References:
SHU-WEI CHANG ET AL: "Codon Optimization of Candida rugosa lip 1 Gene for Improving Expression in Pichia pastoris and Biochemical Characterization of the Purified Recombinant LIP1 Lipase", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 54, no. 3, 1 February 2006 (2006-02-01), pages 815 - 822, XP055039929, ISSN: 0021-8561, DOI: 10.1021/jf052183k
SHU-WEI CHANG ET AL: "Efficient Production of Active Recombinant Candida rugosa LIP3 Lipase in Pichia pastoris and Biochemical Characterization of the Purified Enzyme", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 54, no. 16, 1 August 2006 (2006-08-01), pages 5831 - 5838, XP055382925, ISSN: 0021-8561, DOI: 10.1021/jf060835e
XIAO-XING PAN ET AL: "Efficient Display of Active Geotrichum sp. Lipase on Pichia pastoris Cell Wall and Its Application as a Whole-Cell Biocatalyst To Enrich EPA and DHA in Fish Oil", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 60, no. 38, 26 September 2012 (2012-09-26), US, pages 9673 - 9679, XP055356130, ISSN: 0021-8561, DOI: 10.1021/jf301827y
TSUYOSHI ARAI ET AL: "Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12", FEMS MICROBIOLOGY LETTERS, vol. 152, no. 1, 17 July 1997 (1997-07-17), GB, pages 183 - 188, XP055383252, ISSN: 0378-1097, DOI: 10.1111/j.1574-6968.1997.tb10426.x
SHU-WEI CHANG ET AL: "Efficient Production of Active Recombinant Candida rugosa LIP3 Lipase in Pichia pastoris and Biochemical Characterization of the Purified Enzyme", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 54, no. 16, 1 August 2006 (2006-08-01), pages 5831 - 5838, XP055382925, ISSN: 0021-8561, DOI: 10.1021/jf060835e
XIAO-XING PAN ET AL: "Efficient Display of Active Geotrichum sp. Lipase on Pichia pastoris Cell Wall and Its Application as a Whole-Cell Biocatalyst To Enrich EPA and DHA in Fish Oil", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 60, no. 38, 26 September 2012 (2012-09-26), US, pages 9673 - 9679, XP055356130, ISSN: 0021-8561, DOI: 10.1021/jf301827y
TSUYOSHI ARAI ET AL: "Cloning and sequencing of the cDNA encoding lipase I from Trichosporon fermentans WU-C12", FEMS MICROBIOLOGY LETTERS, vol. 152, no. 1, 17 July 1997 (1997-07-17), GB, pages 183 - 188, XP055383252, ISSN: 0378-1097, DOI: 10.1111/j.1574-6968.1997.tb10426.x
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