PATHOLOGICAL SPECIMEN, METHOD FOR PRODUCING PATHOLOGICAL SPECIMEN, AND METHOD FOR ACQUIRING FLUORESCENCE IMAGE

Title:

PATHOLOGICAL SPECIMEN, METHOD FOR PRODUCING PATHOLOGICAL SPECIMEN, AND METHOD FOR ACQUIRING FLUORESCENCE IMAGE

Document Type and Number:

WIPO Patent Application WO/2016/203952

Kind Code:

A1

Abstract:

[Problem] To provide a means for increasing the sensitivity of a fluorescent signal coming from fluorescent particles in a pathological specimen, for stabilizing the fluorescent signal, and for improving the accuracy of extraction of information from a fluorescence image. [Solution] A pathological specimen comprising: a tissue section which has been subjected to a treatment (an immunostaining treatment or a FISH staining treatment) for fluorescently labeling a biological substance of interest with fluorescent particles which can be observed in a dark field, in accordance with an immunostaining method or a FISH method; a filling layer which coats the tissue section; and a protective layer which coats the filling layer. In the pathological specimen, the refractive index (measurement wavelength = 589 nm, measurement temperature = 20ºC) of each of the fluorescent particles, the filling layer and the protective layer satisfies the requirements represented by the following formulae (1) and (2). (1) |n1-n2| ≤ 0.20 (2) |n2-n3| ≤ 0.15 n1: a refractive index of the fluorescent particles n2: a refractive index of the filling layer n3: a refractive index of the protective layer

Inventors:

WATANABE YASUHIRO (JP)

Application Number:

PCT/JP2016/066197

Publication Date:

December 22, 2016

Filing Date:

June 01, 2016

Export Citation:

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Assignee:

KONICA MINOLTA INC (JP)

International Classes:

G01N33/48; G01N1/28; G01N1/30; G01N21/64; G01N33/53; G01N33/533

Domestic Patent References:

WO2014136776A1

2014-09-12

Foreign References:

JP2015059806A	2015-03-30
JPH0735357A	1995-02-07
JP2012532846A	2012-12-20
JP2012205572A	2012-10-25

Other References: