PCR SUBSTRATE, CHIP AND SYSTEM, AND DROPLET PULL-OUT METHOD

WIPO Patent Application WO/2021/004399

A1

Provided are a PCR substrate, a PCR chip, a PCR system and a droplet pull-out method. The PCR substrate comprises: a first base; and a driving structure arranged on the first base and used for driving droplets to move, wherein the first base comprises an injection region, a stretching region and an amplification region, and the driving structure is used for causing liquid in the injection region to form droplets in the stretching region and causing the droplets to move in the amplification region according to a predetermined trajectory. The driving structure comprises a plurality of driving electrodes for forming an electric field to drive the droplets to move. The PCR chip comprises: the PCR substrate and a sealing substrate facing the driving structure, wherein the sealing structure comprises a second base and a common electrode located on one side, facing the first base, of the second base; an edge region, opposite the sealing substrate, of the PCR substrate is sealed by a sealing member; and an orthographic projection of the sealing member on the first base surrounds orthographic projections of the various driving electrodes on the first base. The PCR chip further comprises a sample inlet hole and a sample outlet hole, which are in communication with a region corresponding to the injection region. The PCR system comprises: the PCR chip or the PCR substrate; a temperature control structure for controlling the temperature at different positions on the predetermined trajectory; and a collection unit for collecting images of the droplets in order to analyze the number of specific basic groups. The droplet pull-out method comprises: injecting a sample into the injection region; stretching the sample from the injection region to the stretching region to obtain a strip-type sample; and cutting off the strip-type sample to form droplets.

JP2023055587	INSPECTION DEVICE AND INSPECTION SYSTEM
JP2007120399	MICRO FLUID CHIP AND MICRO COMPREHENSIVE ANALYSIS SYSTEM
JP2006223312	AUTOMATED PERFORMANCE APPARATUS OF POLYMERASE CHAIN REACTION WITH TEMPERATURE CONTROL

YAO WENLIANG (CN)
CAI PEIZHI (CN)
ZHAO YINGYING (CN)
GU LE (CN)
ZHAO NAN (CN)
CUI HAOCHEN (CN)

PCT/CN2020/100229

January 14, 2021

July 03, 2020

Click for automatic bibliography generation  

BOE TECHNOLOGY GROUP CO LTD (CN)
BEIJING BOE OPTOELECTRONICS TECH CO LTD (CN)

C12M1/38; C12M1/00; C12M1/36

CN108034703A	2018-05-15
CN106497774A	2017-03-15
CN106591109A	2017-04-26
CN106824313A	2017-06-13
CN107904163A	2018-04-13
CN107129933A	2017-09-05

TEE&HOWE INTELLECTUAL PROPERTY ATTORNEYS (CN)

View/Download PDF      PDF Help

Previous Patent: MOTION DATA MONITORING METHOD AND APPARATUS

Next Patent: IMMUNE CELL EXPRESSING CD3 ANTIBODY-RECEPTOR COMPLEX AND USE THEREOF