PD-1 CAR NK-92 CELL AND PREPARATION METHOD THEREFOR AND USE THEREOF

Title:

PD-1 CAR NK-92 CELL AND PREPARATION METHOD THEREFOR AND USE THEREOF

Document Type and Number:

WIPO Patent Application WO/2018/076391

Kind Code:

A1

Abstract:

Provided are a PD-1 CAR NK-92 cell and a preparation method therefor and the use thereof. The PD-1 CAR NK-92 cell expresses a PD-1-CD8TM-4-1BB-CD3 ζ fusion protein in NK-92 cells. The PD-1 CAR NK-92 is obtained by infecting an NK92 cell strain with a PD-1 CAR molecule and obtaining monoclonal cells by means of flow screening, and culturing and amplifying CAR NK92 monoclonal cell strains with stable traits and a high killing activity. The cell can be used in large-scale production and manufacture, can be used for different patients and does not produce GVHR rejection. The killing activity thereof is specific, and the therapeutic effects on tumours are evident.

Inventors:

LI HUASHUN (CN)
WANG BAOLEI (CN)
HAN KUNKUN (CN)
REN BAOYONG (CN)

Application Number:

PCT/CN2016/104493

Publication Date:

May 03, 2018

Filing Date:

November 04, 2016

Export Citation:

Click for automatic bibliography generation Help

Assignee:

LI HUASHUN (CN)

International Classes:

C12N5/10; A61K35/17; A61K48/00; A61P35/00; C07K19/00; C12N7/01; C12N15/62; C12N15/86

Domestic Patent References:

WO2015142675A2	2015-09-24
WO2015090229A1	2015-06-25

Foreign References:

CN105392888A	2016-03-09
CN105132445A	2015-12-09
CN104114233A	2014-10-22

Other References:

CAMPBELL, K. S. ET AL.: "Natural killer cell biology: An update and future directions", JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, vol. 132, no. 3, 30 July 2013 (2013-07-30), pages 536 - 544, XP055145468
GUO, ZHENHONG ET AL.: "The current situation and prospect of immunocyte-therapy for tumor", CHINESE JOURNAL OF CANCER BIOTHERAPY, vol. 23, no. 2, 30 April 2016 (2016-04-30), pages 149 - 160

Attorney, Agent or Firm:

BRIGHTHEAD INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)

Download PDF:

View/Download PDF PDF Help

Previous Patent: WEAR-RESISTANT NOISE-REDUCTION AUTOMOBILE HALF SHAFT

Next Patent: PEDESTRIAN STATISTICAL METHOD AND APPARATUS BASED ON RECOGNITION OF PARIETAL REGION OF HUMAN BODY