Title:
PEGYLATED ERYTHROPOIETIN CONJUGATE AND PREPARATION METHOD AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO/2009/079911
Kind Code:
A1
Abstract:
Provided is a pegylated erythropoietin conjugate having the general formula of rhuEPO-NH-CH2-X-S-Y-mPEG. Also provided is a preparation method of the conjugate comprising carrying out reductive amination on erythropoietin and aldehyde compound containing protected sulfhydryl to form activated erythropoietin linked to -NH-CH2- bond, then carrying out methoxypolyethylene glycol derivative coupling reaction, thus obtaining the conjugate. The conjugate or pharmaceutical composition comprising the conjugate can be used for the treatment of diseases involving erythropoietin insufficiency or red blood cell insufficiency or defect.
Inventors:
LUE AIFENG (CN)
SUN CHANGAN (CN)
WANG RUIJUN (CN)
CHEN KERAN (CN)
LI YUNBO (CN)
WANG YALI (CN)
SUN CHANGAN (CN)
WANG RUIJUN (CN)
CHEN KERAN (CN)
LI YUNBO (CN)
WANG YALI (CN)
Application Number:
PCT/CN2008/001921
Publication Date:
July 02, 2009
Filing Date:
November 25, 2008
Export Citation:
Assignee:
JIANGSU HANSEN PHARMACEUTICAL (CN)
LUE AIFENG (CN)
SUN CHANGAN (CN)
WANG RUIJUN (CN)
CHEN KERAN (CN)
LI YUNBO (CN)
WANG YALI (CN)
LUE AIFENG (CN)
SUN CHANGAN (CN)
WANG RUIJUN (CN)
CHEN KERAN (CN)
LI YUNBO (CN)
WANG YALI (CN)
International Classes:
C07K17/08; A61K47/48; A61P7/06; A61P11/00; A61P13/12; A61P25/00; A61P35/00; A61P37/00; C07K14/505
Foreign References:
| CN1359392A | 2002-07-17 | |||
| CN1680449A | 2005-10-12 | |||
| US20040122164A1 | 2004-06-24 |
Other References:
ROBERTS, M. J. ET AL.: "Chemistry for peptide and protein PEGylation.", ADVANCED DRUG DELIVERY REVIEWS., vol. 54, no. 4, 2002, pages 459 - 476, XP002354432, DOI: doi:10.1016/S0169-409X(02)00022-4
FU, Z. M. ET AL.: "The research progress of protein and peptide site-PEGylation.", BULLETIN OF THE ACADEMY OF MILITARY MEDICAL SCIENCES., vol. 31, no. 2, April 2007 (2007-04-01), pages 178 - 182
FU, Z. M. ET AL.: "The research progress of protein and peptide site-PEGylation.", BULLETIN OF THE ACADEMY OF MILITARY MEDICAL SCIENCES., vol. 31, no. 2, April 2007 (2007-04-01), pages 178 - 182
Attorney, Agent or Firm:
GE CHENG & CO., LTD. (Tower E3 The Towers, Oriental Plaza,No.1, East Chang An Avenue, Dongcheng District, Beijing 8, CN)
Download PDF: