Title:
PENICILLIN G ACYLASE MUTANT
Document Type and Number:
WIPO Patent Application WO/2017/143944
Kind Code:
A1
Abstract:
A penicillin G acylase mutant constructed by means of gene engineering; when compared with wild-type penicillin G acylase derived from Achromobacter sp.CCM 4824, the synthesis performance of the present penicillin G acylase mutant is greatly improved, the synthesis product/hydrolysis product value S/H reaching a maximum of 22.3, 3.9 times that of the wild-type enzyme, being able to efficiently catalyse the synthesis of various β-lactam antibiotics. When the ratio of the side chain to the mother nucleus is 1.05:1, the conversion rate of the 6-APA, 7-ADCA, 7-ACCA, and 7-APRA of the mother nucleus is above 99.0%.
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Inventors:
WANG JINGANG (CN)
LIANG YAN (CN)
CHEN SHUMING (CN)
LIANG YAN (CN)
CHEN SHUMING (CN)
Application Number:
PCT/CN2017/074028
Publication Date:
August 31, 2017
Filing Date:
February 19, 2017
Export Citation:
Assignee:
SHANGHAI GENEWELL BIOTECHNOLOGY CO LTD (CN)
SHANXI XINBAOYUAN PHARMACEUTICAL CO LTD (CN)
SHANXI XINBAOYUAN PHARMACEUTICAL CO LTD (CN)
International Classes:
C12N9/84; C12N15/55; C12P35/04; C12R1/025
Foreign References:
| CN105483105A | 2016-04-13 | |||
| CN102264904A | 2011-11-30 | |||
| CN105274082A | 2016-01-27 | |||
| CN1464055A | 2003-12-31 |
Other References:
DATABASE GenBank 1 September 2005 (2005-09-01), Database accession no. MY 25991.1
PANIGRAHI, P. ET AL.: "Sequenced and Structure-Based Comparative Analysis to Assess, Identify and Improve the Thermostability of Penicillin G Acylases", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 42, no. 11, 30 November 2015 (2015-11-30), pages 1493 - 1506, XP035602983, ISSN: 1367-5435
PANIGRAHI, P. ET AL.: "Sequenced and Structure-Based Comparative Analysis to Assess, Identify and Improve the Thermostability of Penicillin G Acylases", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 42, no. 11, 30 November 2015 (2015-11-30), pages 1493 - 1506, XP035602983, ISSN: 1367-5435
Attorney, Agent or Firm:
SUNHOLD LAW FIRM (CN)
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