Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
PENICILLIN G ACYLASE MUTANT
Document Type and Number:
WIPO Patent Application WO/2017/143944
Kind Code:
A1
Abstract:
A penicillin G acylase mutant constructed by means of gene engineering; when compared with wild-type penicillin G acylase derived from Achromobacter sp.CCM 4824, the synthesis performance of the present penicillin G acylase mutant is greatly improved, the synthesis product/hydrolysis product value S/H reaching a maximum of 22.3, 3.9 times that of the wild-type enzyme, being able to efficiently catalyse the synthesis of various β-lactam antibiotics. When the ratio of the side chain to the mother nucleus is 1.05:1, the conversion rate of the 6-APA, 7-ADCA, 7-ACCA, and 7-APRA of the mother nucleus is above 99.0%.

Inventors:
WANG, Jingang (Bldg 9, No. 230 Chuanhong Road Pudong New District, Shanghai 2, 201202, CN)
LIANG, Yan (Bldg 9, No. 230 Chuanhong Road Pudong New District, Shanghai 2, 201202, CN)
CHEN, Shuming (Bldg 9, No. 230 Chuanhong Road Pudong New District, Shanghai 2, 201202, CN)
Application Number:
CN2017/074028
Publication Date:
August 31, 2017
Filing Date:
February 19, 2017
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
SHANGHAI GENEWELL BIOTECHNOLOGY CO., LTD (Bldg 9, No. 230 Chuanhong Road Pudong New District, Shanghai 2, 201202, CN)
SHANXI XINBAOYUAN PHARMACEUTICAL CO., LTD (Huayuantun Industrial Park, Xinrong DistrictDatong, Shanxi 2, 037002, CN)
International Classes:
C12N9/84; C12N15/55; C12P35/04; C12R1/025
Foreign References:
CN105483105A2016-04-13
CN102264904A2011-11-30
CN105274082A2016-01-27
CN1464055A2003-12-31
Other References:
DATABASE GenBank 1 September 2005 (2005-09-01), Database accession no. MY 25991.1
PANIGRAHI, P. ET AL.: "Sequenced and Structure-Based Comparative Analysis to Assess, Identify and Improve the Thermostability of Penicillin G Acylases", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 42, no. 11, 30 November 2015 (2015-11-30), pages 1493 - 1506, XP035602983, ISSN: 1367-5435
Attorney, Agent or Firm:
SUNHOLD LAW FIRM (16F Haoran High-tech Building, SJTU No. 1954 Huashan Road, Shanghai 0, 200030, CN)
Download PDF: