Title:
PEPTIDE-HINGE-FREE FLEXIBLE ANTIBODY-LIKE MOLECULE
Document Type and Number:
WIPO Patent Application WO/2013/065343
Kind Code:
A1
Abstract:
Provided is an antibody capable of binding to a target with higher affinity. A peptide-hinge-free flexible antibody-like molecule containing a peptide-hinge-free group represented by general formula (I): XY-Asp-Lys-Thr-His-Thr (SEQ ID NO: 1) (in the formula, X represents a peptide constructed from 2-50 amino acid residues or an amino acid, and Y represents an alkylene oxide-containing group) and an antibody Fc fragment bound to this peptide-hinge-free group.
Inventors:
TANAKA KOICHI (JP)
SATO TAKA-AKI (JP)
CAPON DANIEL J (US)
SATO TAKA-AKI (JP)
CAPON DANIEL J (US)
Application Number:
PCT/JP2012/061529
Publication Date:
May 10, 2013
Filing Date:
May 01, 2012
Export Citation:
Assignee:
SHIMADZU CORP (JP)
TANAKA KOICHI (JP)
SATO TAKA-AKI (JP)
CAPON DANIEL J (US)
TANAKA KOICHI (JP)
SATO TAKA-AKI (JP)
CAPON DANIEL J (US)
International Classes:
C07K7/06; C07K16/00; C07K19/00; C12N15/02; C12P21/08
Domestic Patent References:
| WO2007108152A1 | 2007-09-27 | |||
| WO2008140477A2 | 2008-11-20 |
Foreign References:
| JP2011526792A | 2011-10-20 | |||
| JPH10501701A | 1998-02-17 | |||
| JPH07501451A | 1995-02-16 |
Other References:
PAULING, L.: "A theory of the structure and process of formation of antibodies", J. AM. CHEM. SOC., vol. 62, 1940, pages 2643 - 2657
PORTER, R.R.: "Separation and isolation of fractions of rabbit gamma-globulin containing the antibody and antigenic combining sites", NATURE, vol. 182, 1958, pages 670 - 671
EDELMAN, G.M.; CUNNINGHAM, B.A.; GALL, W.E.; GOTTLIEB, P.D.; RUTISHAUSER, U.; WAXDAL, M.J.: "The covalent structure of an entire Gimmunoglobulin molecule", PROC. NATL. ACAD. SCI. U.S.A., vol. 63, 1969, pages 78 - 85
FEINSTEIN, A.; ROWE, A.J.: "Molecular mechanism of formation of an antigen-antibody complex", NATURE, vol. 205, 1965, pages 147 - 149
VALENTINE, R.C.; GREEN, N.M.: "Electron microscopy of an antibody hapten complex", J. MOL. BIOL., vol. 27, 1967, pages 615 - 617
SAPHIRE, E.O.; STANFIELD, R.L.; CRISPIN, M.D.; PARREN, P.W.; RUDD, P.M.; DWEK, R.A.; BURTON, D.R.; WILSON, I.A.: "Contrasting IgG structures reveal extreme asymmetry and flexibility", J. MOL. BIOL., vol. 319, 2002, pages 9 - 18
CAPON, D.J.; CHAMOW, S.M.; MORDENTI, J.; MARSTERS, S.A.; GREGORY, T.; MITSUYA, H.; BYRN, R.A.; LUCAS, C.; WURM, F.M.; GROOPMAN, J.: "Designing CD4 immunoadhesins for AIDS therapy", NATURE, vol. 337, 1989, pages 525 - 531
BYRN, R.A.; MORDENTI, J.; LUCAS, C.; SMITH, D.; MARSTERS, S.A.; JOHNSON, J.S.; COSSUM, P.; CHAMOW, S.M.; WURM, F.M.; GREGORY, T.: "Biological properties of a CD4 immunoadhesin", NATURE, vol. 344, 1990, pages 667 - 670
CHAMOW, S.M.; PEERS, D.H.; BYRN, R.A.; MULKERRIN, M.G.; HARRIS, R.J.; WANG, W.C.; BJORKMAN, P.J.; CAPON, D.J.; ASHKENAZI, A.: "Enzymatic cleavage of a CD4 immunoadhesin generates crystallizable, biologically active Fd-like fragments", BIOCHEMISTRY, vol. 29, 1990, pages 9885 - 9891
WARD, R.H.; CAPON, D.J.; JETT, C.M.; MURTHY, K.K.; MORDENTI, J.; LUCAS, C.; FRIE, S.W.; PRINCE, A.M.; GREEN, J.D.; EICHBERG, J.W.: "Prevention of HIV-1 IIIB infection in chimpanzees by CD4 immunoadhesin", NATURE, vol. 352, 1991, pages 434 - 436
WATSON, S.R.; IMAI, Y.; FENNIE, C.; GEOFFROY, J.S.; ROSEN, S.D.; LASKY, L.A.: "A homing receptor-IgG chimera as a probe for adhesive ligands of lymph node high endothelial venules", J. CELL BIOL., vol. 110, 1990, pages 2221 - 2229
WATSON, S.R.; FENNIE, C.; LASKY, L.A.: "Neutrophil influx into an inflammatory site inhibited by a soluble homing receptor-IgG chimaera", NATURE, vol. 349, 1991, pages 164 - 167
ASHKENAZI, A.; MARSTERS, S.A.; CAPON, D.J.; CHAMOW, S.M.; FIGARI, I.S.; PENNICA, D.; GOEDDEL, D.V.; PALLADINO, M.A.; SMITH, D.H.: "Protection against endotoxic shock by a tumor necrosis factor receptor immunoadhesin", PROC. NATL. ACAD. SCI. U.S.A., vol. 88, 1991, pages 10535 - 10539
ASHKENAZI, A.; CAPON, D.J.; WARD, R.H.: "Immunoadhesins", INT. REV. IMMUNOL., vol. 10, 1993, pages 219 - 227
REICHERT, J.M.: "Antibody-based therapeutics to watch in 2011", MABS, vol. 3, 2011, pages 76 - 99
EDELMAN, G.M.; CUNNINGHAM, B.A.; GALL, W.E.; GOTTLIEB, P.D.; RUTISHAUSER, U.; WAXDAL, M.J.: "The covalent structure of an entire . Gimmunoglobulin molecule", PROC. NATL. ACAD. SCI. U.S.A., vol. 63, 1969, pages 78 - 85
SAPHIRE, E.O.; STANFIELD, R.L.; CRISPIN, M.D; PARREN, P.W.; RUDD, P.M.; DWEK, R.A.; BURTON, D.R.; WILSON, I.A.: "Contrasting IgG structures reveal extreme asymmetry and flexibility", J. MOL. BIOL., vol. 319, 2002, pages 9 - 18
WATSON, S.R.; IMAI, Y.; FENNIE,C.; GEOFFROY, J.S.; ROSEN, S.D.; LASKY, L.A.: "A homing receptor-IgG chimera as a probe for adhesive ligands of lymph node high endothelial venules", J. CELL BIOL., vol. 110, 1990, pages 2221 - 2229
WATSON, S. R.; FENNIE, C.; LASKY, L.A.: "Neutrophil influx into an inflammatory site inhibited by a soluble homing receptor-IgG chimaera", NATURE, vol. 349, 1991, pages 164 - 167
FLORY, P.J.: "Statistical Mechanics of Chain Mole cules", 1969, INTERSCIENCE PUBLISHERS
DAWSON, P.E.; KENT, S.B.: "Synthesis of native proteins by chemical ligation", ANNU. REV. BIOCHEM., vol. 69, 2000, pages 923 - 960
PIRTTILA, T.; KIM, K.S.; MEHTA, P.D.; FREY, H.; WIS NIEWSKI, H.M.: "Soluble amyloid Oprotein in the cerebro spinal fluid from patients with Alzheimer's disease, vascula r dementia and controls", J. NEUROL. SCI., vol. 127, 1994, pages 90 - 95
GARDBERG, A.S.; DICE, L.T.; OU, S.; RICH, R.L.; HELMBR ECHT, E.; KO, J.; WETZEL, R.; MYSZKA, D.G.; PATTERSON, P.H.; DEALWIS, C.: "Molecular basis for passive immunothe rapy of Alzheimer's disease", PROC. NATL. ACAD. SCI. U.S.A., vol. 1, no. 04, 2007, pages 15659 - 15664
ROBERT, R.; DOLEZAL, O.; WADDINGTON, L.; HATTARKI, M.K .; CAPPAI, R.; MASTERS, C.L.; HUDSON, P.J.; WARK, K.L.: "Engineered antibody intervention strategies for Alzheim er's disease and related dementias by targeting amyloid and toxic oligomers", PROTEIN ENG. DES. SEL., vol. 22, 2009, pages 199 - 208
BASI, G.S.; FEINBERG, H.; OSHIDARI, F.; ANDERSON, J.; BARBOUR, R.; BAKER, J.; COMERY, T.A.; DIEP, L.; GILL, D.; JO HNSON-WOOD, K.: "Structural correlates of antibo dies associated with acute reversal of AO-related behavioral deficits in a mouse model of Alzheimer disease", J. BIOL. CH EM., vol. 285, 2010, pages 3417 - 3427
TANAKA, K.; WAKI, H.; IDO, Y.; AKITA, S.; YOSHIDA, Y.; YOSHIDA, T.: "Protein and polymer analyses up to m/z 100,000 by laser ionization time-of-flight mass spectrometr y", RAPID COMMUN. MASS SPECTROM., vol. 2, 1988, pages 151 - 153
KOY, C.; MIKKAT, S.; RAPTAKIS, E.; SUTTON, C.; RESCH, M.; TANAKA, K.; GLOCKER, M.O.: "Matrixassisted laser desorptionlionization- quadrupole ion trap-time of flight m ass spectrometry sequencing resolves structures of unidentif ied peptides obtained by in-gel tryptic digestion of haptogl obin derivatives from human plasma proteomes", PROTEOMICS, vol. 3, 2003, pages 851 - 858
KABAT, E.A.; WU, T.T.; PERRY, H.M.; GOTTESMAN, K.S.; FOELLER, C.: "Sequences of Proteins of Immunological Interest", 1991, NATIONAL INSTITUTES OF HEALTH
RAJENDRA, Y.; KISELJAK, D.; BALDI, L.; HACKER, D.L.; WURM, F.M.: "A simple high-yielding process for trans ient gene expression in CHO cells", J. BIOTECHNOL., vol. 153, 2011, pages 22 - 26
JOHNSON, E.C.; KENT, S.B.: "Insights into the mechanism and catalysis of the native chemical ligation reac tion", J. AM. CHEM. SOC., vol. 128, 2006, pages 6640 - 6646
LUHRS, T.; RITTER, C.; ADRIAN, M.; RIEK-LOHER, D.; BOH RMANN, B.; DOBELI, H.; SCHUBERT, D.; RIEK, R.: "3D s tructure of Alzheimer's A? (1-42) fibrils", PROC. NATL. ACAD. SCI. U.S.A., vol. 102, 2005, pages 17342 - 17347
See also references of EP 2784080A4
PORTER, R.R.: "Separation and isolation of fractions of rabbit gamma-globulin containing the antibody and antigenic combining sites", NATURE, vol. 182, 1958, pages 670 - 671
EDELMAN, G.M.; CUNNINGHAM, B.A.; GALL, W.E.; GOTTLIEB, P.D.; RUTISHAUSER, U.; WAXDAL, M.J.: "The covalent structure of an entire Gimmunoglobulin molecule", PROC. NATL. ACAD. SCI. U.S.A., vol. 63, 1969, pages 78 - 85
FEINSTEIN, A.; ROWE, A.J.: "Molecular mechanism of formation of an antigen-antibody complex", NATURE, vol. 205, 1965, pages 147 - 149
VALENTINE, R.C.; GREEN, N.M.: "Electron microscopy of an antibody hapten complex", J. MOL. BIOL., vol. 27, 1967, pages 615 - 617
SAPHIRE, E.O.; STANFIELD, R.L.; CRISPIN, M.D.; PARREN, P.W.; RUDD, P.M.; DWEK, R.A.; BURTON, D.R.; WILSON, I.A.: "Contrasting IgG structures reveal extreme asymmetry and flexibility", J. MOL. BIOL., vol. 319, 2002, pages 9 - 18
CAPON, D.J.; CHAMOW, S.M.; MORDENTI, J.; MARSTERS, S.A.; GREGORY, T.; MITSUYA, H.; BYRN, R.A.; LUCAS, C.; WURM, F.M.; GROOPMAN, J.: "Designing CD4 immunoadhesins for AIDS therapy", NATURE, vol. 337, 1989, pages 525 - 531
BYRN, R.A.; MORDENTI, J.; LUCAS, C.; SMITH, D.; MARSTERS, S.A.; JOHNSON, J.S.; COSSUM, P.; CHAMOW, S.M.; WURM, F.M.; GREGORY, T.: "Biological properties of a CD4 immunoadhesin", NATURE, vol. 344, 1990, pages 667 - 670
CHAMOW, S.M.; PEERS, D.H.; BYRN, R.A.; MULKERRIN, M.G.; HARRIS, R.J.; WANG, W.C.; BJORKMAN, P.J.; CAPON, D.J.; ASHKENAZI, A.: "Enzymatic cleavage of a CD4 immunoadhesin generates crystallizable, biologically active Fd-like fragments", BIOCHEMISTRY, vol. 29, 1990, pages 9885 - 9891
WARD, R.H.; CAPON, D.J.; JETT, C.M.; MURTHY, K.K.; MORDENTI, J.; LUCAS, C.; FRIE, S.W.; PRINCE, A.M.; GREEN, J.D.; EICHBERG, J.W.: "Prevention of HIV-1 IIIB infection in chimpanzees by CD4 immunoadhesin", NATURE, vol. 352, 1991, pages 434 - 436
WATSON, S.R.; IMAI, Y.; FENNIE, C.; GEOFFROY, J.S.; ROSEN, S.D.; LASKY, L.A.: "A homing receptor-IgG chimera as a probe for adhesive ligands of lymph node high endothelial venules", J. CELL BIOL., vol. 110, 1990, pages 2221 - 2229
WATSON, S.R.; FENNIE, C.; LASKY, L.A.: "Neutrophil influx into an inflammatory site inhibited by a soluble homing receptor-IgG chimaera", NATURE, vol. 349, 1991, pages 164 - 167
ASHKENAZI, A.; MARSTERS, S.A.; CAPON, D.J.; CHAMOW, S.M.; FIGARI, I.S.; PENNICA, D.; GOEDDEL, D.V.; PALLADINO, M.A.; SMITH, D.H.: "Protection against endotoxic shock by a tumor necrosis factor receptor immunoadhesin", PROC. NATL. ACAD. SCI. U.S.A., vol. 88, 1991, pages 10535 - 10539
ASHKENAZI, A.; CAPON, D.J.; WARD, R.H.: "Immunoadhesins", INT. REV. IMMUNOL., vol. 10, 1993, pages 219 - 227
REICHERT, J.M.: "Antibody-based therapeutics to watch in 2011", MABS, vol. 3, 2011, pages 76 - 99
EDELMAN, G.M.; CUNNINGHAM, B.A.; GALL, W.E.; GOTTLIEB, P.D.; RUTISHAUSER, U.; WAXDAL, M.J.: "The covalent structure of an entire . Gimmunoglobulin molecule", PROC. NATL. ACAD. SCI. U.S.A., vol. 63, 1969, pages 78 - 85
SAPHIRE, E.O.; STANFIELD, R.L.; CRISPIN, M.D; PARREN, P.W.; RUDD, P.M.; DWEK, R.A.; BURTON, D.R.; WILSON, I.A.: "Contrasting IgG structures reveal extreme asymmetry and flexibility", J. MOL. BIOL., vol. 319, 2002, pages 9 - 18
WATSON, S.R.; IMAI, Y.; FENNIE,C.; GEOFFROY, J.S.; ROSEN, S.D.; LASKY, L.A.: "A homing receptor-IgG chimera as a probe for adhesive ligands of lymph node high endothelial venules", J. CELL BIOL., vol. 110, 1990, pages 2221 - 2229
WATSON, S. R.; FENNIE, C.; LASKY, L.A.: "Neutrophil influx into an inflammatory site inhibited by a soluble homing receptor-IgG chimaera", NATURE, vol. 349, 1991, pages 164 - 167
FLORY, P.J.: "Statistical Mechanics of Chain Mole cules", 1969, INTERSCIENCE PUBLISHERS
DAWSON, P.E.; KENT, S.B.: "Synthesis of native proteins by chemical ligation", ANNU. REV. BIOCHEM., vol. 69, 2000, pages 923 - 960
PIRTTILA, T.; KIM, K.S.; MEHTA, P.D.; FREY, H.; WIS NIEWSKI, H.M.: "Soluble amyloid Oprotein in the cerebro spinal fluid from patients with Alzheimer's disease, vascula r dementia and controls", J. NEUROL. SCI., vol. 127, 1994, pages 90 - 95
GARDBERG, A.S.; DICE, L.T.; OU, S.; RICH, R.L.; HELMBR ECHT, E.; KO, J.; WETZEL, R.; MYSZKA, D.G.; PATTERSON, P.H.; DEALWIS, C.: "Molecular basis for passive immunothe rapy of Alzheimer's disease", PROC. NATL. ACAD. SCI. U.S.A., vol. 1, no. 04, 2007, pages 15659 - 15664
ROBERT, R.; DOLEZAL, O.; WADDINGTON, L.; HATTARKI, M.K .; CAPPAI, R.; MASTERS, C.L.; HUDSON, P.J.; WARK, K.L.: "Engineered antibody intervention strategies for Alzheim er's disease and related dementias by targeting amyloid and toxic oligomers", PROTEIN ENG. DES. SEL., vol. 22, 2009, pages 199 - 208
BASI, G.S.; FEINBERG, H.; OSHIDARI, F.; ANDERSON, J.; BARBOUR, R.; BAKER, J.; COMERY, T.A.; DIEP, L.; GILL, D.; JO HNSON-WOOD, K.: "Structural correlates of antibo dies associated with acute reversal of AO-related behavioral deficits in a mouse model of Alzheimer disease", J. BIOL. CH EM., vol. 285, 2010, pages 3417 - 3427
TANAKA, K.; WAKI, H.; IDO, Y.; AKITA, S.; YOSHIDA, Y.; YOSHIDA, T.: "Protein and polymer analyses up to m/z 100,000 by laser ionization time-of-flight mass spectrometr y", RAPID COMMUN. MASS SPECTROM., vol. 2, 1988, pages 151 - 153
KOY, C.; MIKKAT, S.; RAPTAKIS, E.; SUTTON, C.; RESCH, M.; TANAKA, K.; GLOCKER, M.O.: "Matrixassisted laser desorptionlionization- quadrupole ion trap-time of flight m ass spectrometry sequencing resolves structures of unidentif ied peptides obtained by in-gel tryptic digestion of haptogl obin derivatives from human plasma proteomes", PROTEOMICS, vol. 3, 2003, pages 851 - 858
KABAT, E.A.; WU, T.T.; PERRY, H.M.; GOTTESMAN, K.S.; FOELLER, C.: "Sequences of Proteins of Immunological Interest", 1991, NATIONAL INSTITUTES OF HEALTH
RAJENDRA, Y.; KISELJAK, D.; BALDI, L.; HACKER, D.L.; WURM, F.M.: "A simple high-yielding process for trans ient gene expression in CHO cells", J. BIOTECHNOL., vol. 153, 2011, pages 22 - 26
JOHNSON, E.C.; KENT, S.B.: "Insights into the mechanism and catalysis of the native chemical ligation reac tion", J. AM. CHEM. SOC., vol. 128, 2006, pages 6640 - 6646
LUHRS, T.; RITTER, C.; ADRIAN, M.; RIEK-LOHER, D.; BOH RMANN, B.; DOBELI, H.; SCHUBERT, D.; RIEK, R.: "3D s tructure of Alzheimer's A? (1-42) fibrils", PROC. NATL. ACAD. SCI. U.S.A., vol. 102, 2005, pages 17342 - 17347
See also references of EP 2784080A4
Attorney, Agent or Firm:
OKADA, MASAHIRO (JP)
Masahiro Okada (JP)
Masahiro Okada (JP)
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