Title:
PEPTIDE PURIFICATION BY MEANS OF HARD METAL ION AFFINITY CHROMATOGRAPHY
Document Type and Number:
WIPO Patent Application WO2005120700
Kind Code:
A3
Abstract:
A polymer substrate functionalized with a functionality comprising at least one cyclic, metal ion coordinating ligand group which comprises at least 3 nitrogen donor atoms in the ring of the cyclic group, at least one of the nitrogen atoms having an optionally substituted carboxy(lower alkyl) or optionally substituted phosphono(lower alkyl) group covalently attached thereto, is well suited for use in conjunction with "hard" metal ions of low toxicity (such as Ca2+, Mg2+or Fe3+) in the separation/purification of appropriately "tagged" polypeptides byImmobilized Metal ion Affinity Chromatography (IMAC).
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Inventors:
HEARN MILTON THOMAS WILLIAM (AU)
SPICCIA LEONE (AU)
DALY RACHEL (AU)
KREHER UTE (AU)
SPICCIA LEONE (AU)
DALY RACHEL (AU)
KREHER UTE (AU)
Application Number:
PCT/EP2005/052745
Publication Date:
July 20, 2006
Filing Date:
June 14, 2005
Export Citation:
Assignee:
NOVO NORDISK AS (DK)
UNIV MONASH (AU)
HEARN MILTON THOMAS WILLIAM (AU)
SPICCIA LEONE (AU)
DALY RACHEL (AU)
KREHER UTE (AU)
UNIV MONASH (AU)
HEARN MILTON THOMAS WILLIAM (AU)
SPICCIA LEONE (AU)
DALY RACHEL (AU)
KREHER UTE (AU)
International Classes:
C07K17/10; B01D15/38; B01J20/26; B01J45/00; C07K1/22; C07K7/06; C07K7/08; C12N15/63; C12P21/02
Domestic Patent References:
| WO2003042249A2 | 2003-05-22 | |||
| WO1992013965A1 | 1992-08-20 | |||
| WO2002082051A2 | 2002-10-17 | |||
| WO1995028966A1 | 1995-11-02 | |||
| WO2005049096A2 | 2005-06-02 |
Other References:
COROT C ET AL: "Physical, chemical and biological evaluations of CMD-A2-Gd-DOTA. A new paramagnetic dextran polymer.", ACTA RADIOLOGICA. SUPPLEMENTUM. 1997, vol. 412, 1997, pages 91 - 99, XP009058210, ISSN: 0001-6926
WEISSLEDER R ET AL: "SIZE OPTIMIZATION OF SYNTHETIC GRAFT COPOLYMERS FOR IN VIVO ANGIOGENESIS IMAGING", BIOCONJUGATE CHEMISTRY, ACS, WASHINGTON, DC, US, vol. 12, no. 2, March 2001 (2001-03-01), pages 213 - 219, XP008051687, ISSN: 1043-1802
JIANG W ET AL: "Protein selectivity with immobilized metal ion-tacn sorbents: chromatographic studies with human serum proteins and several other globular proteins", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS INC. NEW YORK, US, vol. 255, 1998, pages 47 - 58, XP002196669, ISSN: 0003-2697
ROY B C ET AL: "SYNTHESIS OF CONJUGATED DIACETYLENE, METAL-CHELATING MONOMERS FOR POLYMERIZABLE MONOLAYER ASSEMBLIES", ORGANIC LETTERS, ACS, WASHINGTON, DC, US, vol. 3, no. 12, 2001, pages 1877 - 1879, XP001147819, ISSN: 1523-7060
STUDER M ET AL: "SYNTHESIS OF NOVEL 1 4 7 TRIAZACYCLONONANE-N N' N'-TRIACETIC ACID DERIVATIVES SUITABLE FOR PROTEIN LABELING", BIOCONJUGATE CHEMISTRY, vol. 3, no. 4, 1992, pages 337 - 341, XP009058163, ISSN: 1043-1802
HONG J ET AL: "Cloning of a gene encoding thermostable cellobiohydrolase from Thermoascus aurantiacus and its expression in yeast.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. NOV 2003, vol. 63, no. 1, November 2003 (2003-11-01), pages 42 - 50, XP002376333, ISSN: 0175-7598
WEISSLEDER R ET AL: "SIZE OPTIMIZATION OF SYNTHETIC GRAFT COPOLYMERS FOR IN VIVO ANGIOGENESIS IMAGING", BIOCONJUGATE CHEMISTRY, ACS, WASHINGTON, DC, US, vol. 12, no. 2, March 2001 (2001-03-01), pages 213 - 219, XP008051687, ISSN: 1043-1802
JIANG W ET AL: "Protein selectivity with immobilized metal ion-tacn sorbents: chromatographic studies with human serum proteins and several other globular proteins", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS INC. NEW YORK, US, vol. 255, 1998, pages 47 - 58, XP002196669, ISSN: 0003-2697
ROY B C ET AL: "SYNTHESIS OF CONJUGATED DIACETYLENE, METAL-CHELATING MONOMERS FOR POLYMERIZABLE MONOLAYER ASSEMBLIES", ORGANIC LETTERS, ACS, WASHINGTON, DC, US, vol. 3, no. 12, 2001, pages 1877 - 1879, XP001147819, ISSN: 1523-7060
STUDER M ET AL: "SYNTHESIS OF NOVEL 1 4 7 TRIAZACYCLONONANE-N N' N'-TRIACETIC ACID DERIVATIVES SUITABLE FOR PROTEIN LABELING", BIOCONJUGATE CHEMISTRY, vol. 3, no. 4, 1992, pages 337 - 341, XP009058163, ISSN: 1043-1802
HONG J ET AL: "Cloning of a gene encoding thermostable cellobiohydrolase from Thermoascus aurantiacus and its expression in yeast.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. NOV 2003, vol. 63, no. 1, November 2003 (2003-11-01), pages 42 - 50, XP002376333, ISSN: 0175-7598
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