Title:
PEPTIDE PURIFICATION BY MEANS OF METAL ION AFFINITY CHROMATOGRAPHY
Document Type and Number:
WIPO Patent Application WO2003042249
Kind Code:
A8
Abstract:
A polymer substrate functionalized with a functionality comprising at least one cyclic, metal ion coordinating ligand group, the cyclic ligand group comprising at least 3 metal ion coordinating donor atoms independently selected from the group consisting of N, O and S. The macrocydic ligand is positioned as a "puckered" face of the metal ion coordination sphere leading to an uni-directional approach and orientation for the incoming oligopeptide "tag" of a "tagged" protein (fusion protein). The polymer substrate serves a matrix in the field of isolation and purification of peptides by means of immobilized metal ion affinity cromatography.
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Inventors:
CHRISTENSEN THORKILD (DK)
HEARN MILTON THOMAS WILLIAM (AU)
SPICCIA LEONE (AU)
JIANG WEI (AU)
MOONEY JANE THERESE (AU)
GRAHAM BIMBIL (AU)
HEARN MILTON THOMAS WILLIAM (AU)
SPICCIA LEONE (AU)
JIANG WEI (AU)
MOONEY JANE THERESE (AU)
GRAHAM BIMBIL (AU)
Application Number:
PCT/DK2002/000758
Publication Date:
April 08, 2004
Filing Date:
November 12, 2002
Export Citation:
Assignee:
NOVO NORDISK AS (DK)
UNIV MONASH (AU)
CHRISTENSEN THORKILD (DK)
HEARN MILTON THOMAS WILLIAM (AU)
SPICCIA LEONE (AU)
JIANG WEI (AU)
MOONEY JANE THERESE (AU)
GRAHAM BIMBIL (AU)
UNIV MONASH (AU)
CHRISTENSEN THORKILD (DK)
HEARN MILTON THOMAS WILLIAM (AU)
SPICCIA LEONE (AU)
JIANG WEI (AU)
MOONEY JANE THERESE (AU)
GRAHAM BIMBIL (AU)
International Classes:
B01D15/08; B01J20/26; B01J20/281; B01J45/00; C07K1/22; C07K2/00; C07K7/08; C07K14/00; C07K19/00; C12N15/09; C08B37/00; C08B37/02; C08F8/00; C08G65/32; C08G65/321; C12N1/21; C12N5/10; C12N15/11; C12N15/62; C12N15/63; C12P21/02; C12P21/06; (IPC1-7): C08B37/00; B01D15/08; C07K1/22; C07K7/08; C07K19/00; C12N15/11; C12N15/62; C12N15/63; C12N5/10
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