Title:
PEPTIDE SPECIFICALLY BINDING TO IGY AND METHOD FOR PURIFYING IGY USING SAME
Document Type and Number:
WIPO Patent Application WO/2015/053353
Kind Code:
A1
Abstract:
Provided is a peptide that specifically binds to IgY.
A peptide characterized by: containing an amino acid sequence that consists of 17 amino acid residues and is represented by a formula: (X1-3)-C-(X5)-W-(X7-11)-W-(X13)-C-(X15-17) [wherein X1 and X3, among X1-3, and X15-17 independently represent an arbitrary amino acid residue other than a cysteine residue; X2 represents an arbitrary amino acid residue that may be a cysteine residue; C represents a cysteine residue; X5 represents an amino acid residue selected from among T, D, V, S and E; W represents a tryptophan residue; X7-11 represents an amino acid sequence (S, T, R or N)-(S, A, K or R)-(I, A, V, T or G)-(V, Y, T, R or S)-(G or D); and X13 represents an amino acid residue selected from among V, L, D, S, M, T and R]; a disulfide bond being formed between cysteine residues at the 4- and 14-positions; and being capable of binding to tri-IgY. A method for analyzing or purifying IgY using the aforesaid peptide
Inventors:
ITO YUJI (JP)
Application Number:
PCT/JP2014/077036
Publication Date:
April 16, 2015
Filing Date:
October 09, 2014
Export Citation:
Assignee:
UNIV KAGOSHIMA (JP)
International Classes:
C07K19/00; C07K7/08; C07K7/64; C07K17/02; C12N15/09; G01N33/53; G01N33/531
Domestic Patent References:
| WO2001083515A2 | 2001-11-08 | |||
| WO2013027796A1 | 2013-02-28 | |||
| WO2013081037A1 | 2013-06-06 |
Foreign References:
| US20060223986A1 | 2006-10-05 |
Other References:
MOLLEGAARD, K.M. ET AL.: "The interactions of calreticulin with immunoglobulin G and immunoglobulin Y", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1814, 2011, pages 889 - 899
CAO, Y. ET AL.: "Characterization of the optimized C2 domain of protein G: finding its additional chicken IgY-binding ability", BIOTECHNOL. LETT., vol. 35, 21 May 2013 (2013-05-21), pages 1441 - 1447
TAN, S.H. ET AL.: "A novel, cost-effective and efficient chicken egg IgY purification procedure", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 380, 2012, pages 73 - 76, XP028488749, DOI: doi:10.1016/j.jim.2012.03.003
HATANAKA, T. ET AL.: "Human IgA-binding Peptides Selected from Random Peptide Libraries", AFFINITY MATURATION AND APPLICATION IN IGA PURIFICATION, THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 287, no. 51, 14 December 2012 (2012-12-14), pages 43126 - 43136, XP055073353, DOI: doi:10.1074/jbc.M112.389742
IMAMURA, A. ET AL.: "Identification of Chicken IgY-Specific Binding Peptide from Random Peptide Library and its Application for IgY Affinity Purification", THE 4TH ASIA-PACIFIC INTERNATIONAL PEPTIDE SYMPOSIUM, THE 50TH JAPANESE PEPTIDE SYMPOSIUM, APIPS2013 KOEN YOSHISHU, 10 October 2013 (2013-10-10)
IMAMURA, A. ET AL.: "Identification of Chicken IgY-Specific Binding Peptide from Random Peptide Library and its Application for IgY Affinity Purification", PEPTIDE SCIENCE 2013, March 2014 (2014-03-01), pages 419 - 420
CAO, Y. ET AL.: "Characterization of the optimized C2 domain of protein G: finding its additional chicken IgY-binding ability", BIOTECHNOL. LETT., vol. 35, 21 May 2013 (2013-05-21), pages 1441 - 1447
TAN, S.H. ET AL.: "A novel, cost-effective and efficient chicken egg IgY purification procedure", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 380, 2012, pages 73 - 76, XP028488749, DOI: doi:10.1016/j.jim.2012.03.003
HATANAKA, T. ET AL.: "Human IgA-binding Peptides Selected from Random Peptide Libraries", AFFINITY MATURATION AND APPLICATION IN IGA PURIFICATION, THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 287, no. 51, 14 December 2012 (2012-12-14), pages 43126 - 43136, XP055073353, DOI: doi:10.1074/jbc.M112.389742
IMAMURA, A. ET AL.: "Identification of Chicken IgY-Specific Binding Peptide from Random Peptide Library and its Application for IgY Affinity Purification", THE 4TH ASIA-PACIFIC INTERNATIONAL PEPTIDE SYMPOSIUM, THE 50TH JAPANESE PEPTIDE SYMPOSIUM, APIPS2013 KOEN YOSHISHU, 10 October 2013 (2013-10-10)
IMAMURA, A. ET AL.: "Identification of Chicken IgY-Specific Binding Peptide from Random Peptide Library and its Application for IgY Affinity Purification", PEPTIDE SCIENCE 2013, March 2014 (2014-03-01), pages 419 - 420
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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