Title:
PEPTIDES CONTAINING RESPECTIVE AMINO ACID SEQUENCES SELECTED FROM AMONG THOSE OF LIPOPROTEIN(a) AND APOLIPOPROTEIN(a), ANTIBODIES RESPECTIVELY RECOGNIZING THESE AMINO ACID SEQUENCES, AND METHOD OF ASSAYING WITH THESE ANTIBODIES
Document Type and Number:
WIPO Patent Application WO/1994/004563
Kind Code:
A1
Abstract:
Amino acid sequences which have a specificity as a lipoprotein(a) and are lowly homologous with LDL and plasminogen are selected from among the amino acid sequences of the lipoprotein(a) to obtain antibodies against the lipoprotein(a) which specifically recognize these amino acid sequences. At least one of these antibodies is used in the immunoassay of the lipoprotein(a). Amino acid sequences which have a specificity as an apolipoprotein(a) and do not have antigenicities as the lipoprotein(a) and plasminogen are selected from among the amino acid sequences of the apolipoprotein(a) to obtain antibodies against the apolipoprotein(a) which specifically recognize these amino acid sequences. At least one of these antibodies is used in the immunoassay of the apolipoprotein(a). The invention serves to prepare antibodies against lipoprotein(a) and apolipoprotein(a) more readily than the conventional methods and to conduct the immunoassay of the lipoprotein(a) and apolipoprotein(a) accurately by using the antibodies.
Inventors:
YAMADA SHINGO (JP)
INOUE KEIICHI (JP)
KITAJIMA MEGUMI (JP)
YOSHIMURA HAJIME (JP)
SAKURABAYASHI IKUNOSUKE (JP)
INOUE KEIICHI (JP)
KITAJIMA MEGUMI (JP)
YOSHIMURA HAJIME (JP)
SAKURABAYASHI IKUNOSUKE (JP)
Application Number:
PCT/JP1993/001142
Publication Date:
March 03, 1994
Filing Date:
August 12, 1993
Export Citation:
Assignee:
SHINO TEST CORP (JP)
YAMADA SHINGO (JP)
INOUE KEIICHI (JP)
KITAJIMA MEGUMI (JP)
YOSHIMURA HAJIME (JP)
SAKURABAYASHI IKUNOSUKE (JP)
YAMADA SHINGO (JP)
INOUE KEIICHI (JP)
KITAJIMA MEGUMI (JP)
YOSHIMURA HAJIME (JP)
SAKURABAYASHI IKUNOSUKE (JP)
International Classes:
C07K14/47; C07K14/775; C07K16/18; G01N33/92; (IPC1-7): C07K7/06; C07K7/08; G01N33/53
Foreign References:
| JP4505609A |
Other References:
Nature (London), Vol. 330, No. 6144 (1987), JOHN W. McLEAN et al., "cDNA sequence of human apolipoprotein (a) is homologous to plasminogen", pp. 132-137.
Biochem. J., Vol. 288, No. 1 (1992), GI CHEN et al., "Studies on epitopes on low-density lipoprotein modified by 4-hydroxynonenal. Biochemical characterization and determination", pp. 249-254.
See also references of EP 0621284A4
Biochem. J., Vol. 288, No. 1 (1992), GI CHEN et al., "Studies on epitopes on low-density lipoprotein modified by 4-hydroxynonenal. Biochemical characterization and determination", pp. 249-254.
See also references of EP 0621284A4
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