Title:
PHAGE DISPLAY AFFINITY AND SPECIFICITY FILTERS AND FORWARD SCREEN
Document Type and Number:
WIPO Patent Application WO2003084981
Kind Code:
A3
Abstract:
The invention provides an affinity filter for the binding of phage-displayed proteins to dissolved target molecules. The phage-displayed proteins are contacted with immobilized target in the presence and absence of dissolved target; the behavior of the phage-displayed proteins as a function of concentration of dissolved target permits approximation of the affinity of the phage-displayed protein for target. The invention also provides a method to screen large numbers of compounds for their ability to complete with a compound known to bind a phage-displayed protein.
Inventors:
LOCKHART DAVID J
TREIBER DANIEL KELLY
ZARRINKAR PATRICK P
TREIBER DANIEL KELLY
ZARRINKAR PATRICK P
Application Number:
PCT/US2003/010247
Publication Date:
December 18, 2003
Filing Date:
April 02, 2003
Export Citation:
Assignee:
AMBIT BIOSCIENCES CORP (US)
International Classes:
C12N15/10; C40B40/02; (IPC1-7): C12Q1/70; C12Q1/68; G01N33/53; C12P21/06; C12N15/00; C07H21/04
Foreign References:
| US6326155B1 | 2001-12-04 |
Other References:
XU WEI ET AL.: "Anti-peptide aptamers recognize amino acid sequence and bind a protein epitope", PROC. NATL. ACAD. SCI. USA, vol. 93, July 1996 (1996-07-01), pages 7475 - 7480, XP002182269
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