Title:
PHARMACEUTICALLY COMPATIBLE METHOD FOR PURIFYING INTACT BACTERIAL MINICELLS
Document Type and Number:
WIPO Patent Application WO2004113507
Kind Code:
A9
Abstract:
The present invention provides a method for purifying bacterial minicells that involves subjecting a sample containing minicells to density gradient centrifugation in a biologically compatible medium. The method optionally includes a preliminary differential centrifugation step and one or more filtration steps. The invention also provides a method for purifying bacterial minicells in which a sample containing minicells is subjected to a condition that induces parent bacterial cells to adopt a filamentous form, followed by filtration of the sample to separate minicells from parent bacterial cells. The inventive methods optionally include one or more steps to remove endotoxin from purified minicell preparations, and/or treatment of purified minicell preparations with an antibiotic. Additionally, the invention provides purified minicell preparations, prepared according to the foregoing methods, and containing fewer than about 1 contaminating parent bacterial cell per 107, 108, 109, 1010, or 1011 minicells.
Inventors:
BRAHMBHATT HIMANSHU (AU)
MACDIARMID JENNIFER (AU)
MACDIARMID JENNIFER (AU)
Application Number:
PCT/IB2004/002091
Publication Date:
August 24, 2006
Filing Date:
June 23, 2004
Export Citation:
Assignee:
ENGENEIC MOLECULAR DELIVERY PTY LTD (AU)
BRAHMBHATT HIMANSHU (AU)
MACDIARMID JENNIFER (AU)
BRAHMBHATT HIMANSHU (AU)
MACDIARMID JENNIFER (AU)
International Classes:
C12N1/02; C12N1/20; C12N5/02
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