Title:
PHOSPHOKETOLASE WITH ENHANCED ACTIVITY AND USE THEREOF IN METABOLITE PRODUCTION
Document Type and Number:
WIPO Patent Application WO/2020/220432
Kind Code:
A1
Abstract:
Disclosed are a phosphoketolase with enhanced activity and a use thereof in metabolite production. A protein is provided, being a mutant protein obtained by subjecting a phosphoketolase to any one or more of the following mutations: T at position 2 is mutated to A; I at position 6 is mutated to T; N at position 14 is mutated to D; E at position 20 is mutated to D; T at position 120 is mutated to A, E at position 231 is mutated to K; H at position 260 is mutated to Y; E at position 342 is mutated to K; K at position 397 is mutated to R, D at position 676 is mutated to G, F at position 785 is mutated to L; and W at position 801 is mutated to R. Also disclosed is a use of the mutant protein in metabolite production. Compared to existing phosphoketolases, the mutant protein phosphoketolase provided has markedly enhanced enzyme activity, which can significantly increase the yield of a target metabolite.
Inventors:
SUN JIBIN (CN)
LI QINGGANG (CN)
ZHENG PING (CN)
ZHOU WENJUAN (CN)
ZHANG XIAOLI (CN)
DELE-OSIBANJO TAIWO ADEOLU (CN)
MA YANHE (CN)
LI QINGGANG (CN)
ZHENG PING (CN)
ZHOU WENJUAN (CN)
ZHANG XIAOLI (CN)
DELE-OSIBANJO TAIWO ADEOLU (CN)
MA YANHE (CN)
Application Number:
PCT/CN2019/090481
Publication Date:
November 05, 2020
Filing Date:
June 10, 2019
Export Citation:
Assignee:
TIANJIN INST IND BIOTECHNOLOGY CAS (CN)
International Classes:
C12N9/00; C12N15/52; C12P7/24; C12P7/26; C12P9/00; C12P19/32
Domestic Patent References:
| WO2011159853A1 | 2011-12-22 |
Foreign References:
| CN101090964A | 2007-12-19 | |||
| CN107893089A | 2018-04-10 | |||
| CN106916794A | 2017-07-04 |
Other References:
NCBI REFERENCE SEQUENCE: WP_011743105.1: "Phosphoketolase [Bifidobacterium adolescentis]", NCBI, 20 August 2018 (2018-08-20), DOI: 20200113162232A
TAKAHASHI, K. ET AL.: "3AI7 Crystal structure of bifidobacterium longum phosphoketolase", RCSB PDB, 15 September 2010 (2010-09-15), XP055751434, DOI: 20200113161817A
ZHAO, J. ET AL.: "A rewiew on research progress of tansketolase", NEUROSCI BULL, vol. 25, no. 2, 1 April 2019 (2019-04-01), XP055751436, DOI: 20200113141007A
TAKAHASHI, K. ET AL.: "3AI7 Crystal structure of bifidobacterium longum phosphoketolase", RCSB PDB, 15 September 2010 (2010-09-15), XP055751434, DOI: 20200113161817A
ZHAO, J. ET AL.: "A rewiew on research progress of tansketolase", NEUROSCI BULL, vol. 25, no. 2, 1 April 2019 (2019-04-01), XP055751436, DOI: 20200113141007A
Attorney, Agent or Firm:
JEEKAI & PARTNERS (CN)
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