Title:
PHOSPHOR AGGREGATE NANOPARTICLE, STAINING REAGENT USING SAME, KIT, AND FLUORESCENT IMMUNOSTAINING METHOD
Document Type and Number:
WIPO Patent Application WO/2015/159776
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide: phosphor aggregate nanoparticles for achieving a fluorescent immunostaining method having higher detection performance; and a staining reagent and fluorescent immunostaining method that use same. Phosphor aggregate nanoparticles are less likely to be non-specifically adsorbed by other living molecules, etc., and detection target (gene or antigen, etc., related to a specific condition) staining efficiency during immunostaining is improved, as a result of using, as the fluorescent component of a staining reagent used in FISH or tissue immunostaining, phosphor aggregate nanoparticles having: dye particles having an average particle diameter of 40-300 nm and comprising phosphors accumulated on the surface of or inside particles that serve as a base; and first living molecules that have a bonding site and are on the surface of the dye particles. The proportion of first living molecules on the surface of the dye particles that have an active bonding site is 0.001-0.036/nm2. The sensitivity and precision of detection of detection targets during fluorescent immunostaining can be increased as a result.
Inventors:
ISODA TAKESHI (JP)
TAKANASHI KENSAKU (JP)
TAKANASHI KENSAKU (JP)
Application Number:
PCT/JP2015/060995
Publication Date:
October 22, 2015
Filing Date:
April 08, 2015
Export Citation:
Assignee:
KONICA MINOLTA INC (JP)
International Classes:
G01N33/533; G01N33/53; G01N33/536; G01N33/545
Domestic Patent References:
| WO2013035688A1 | 2013-03-14 | |||
| WO2012133047A1 | 2012-10-04 | |||
| WO2012029342A1 | 2012-03-08 | |||
| WO2013146694A1 | 2013-10-03 |
Foreign References:
| JP2011083256A | 2011-04-28 |
Attorney, Agent or Firm:
SSINPAT PATENT FIRM (JP)
Patent business corporation SSINPAT (JP)
Patent business corporation SSINPAT (JP)
Download PDF: